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Agrobacterium Protocols

  • Book
  • Jun 1995

Overview

Editors:
  1. Kevan M. A. Gartland

    1. Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Michael R. Davey

    1. Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 44)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Growth and Storage of Agrobacterium

    • Fraukje M. van Asma
    Pages 1-7

  3. Simple Cultural Tests for Identification of Agrobacterium Biovars

    • Hacène Bouzar, Jeffrey B. Jones, Andrew L. Bishop
    Pages 9-13

  4. Agrobacterium Virulence

    • Jill S. Gartland
    Pages 15-28

  5. Agrobacterium tumefaciens Chemotaxis Protocols

    • Charles H. Shaw
    Pages 29-36

  6. Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens

    • Patrice Dion, Christian Bélanger, Dong Xu, Mahmood Mohammadi
    Pages 37-45

  7. Binary Ti Plasmid Vectors

    • Gynheung An
    Pages 47-58

  8. Leaf Disk Transformation

    • Ian S. Curtis, Michael R. Davey, J. Brian Power
    Pages 59-70

  9. Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.)

    • Jürgen E. Thomzik
    Pages 71-78

  10. Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.)

    • Jürgen E. Thomzik
    Pages 79-85

  11. Peanut Transformation

    • Elisabeth Mansur, Cristiano Lacorte, William R. Krul
    Pages 87-100

  12. Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants

    • Paula P. Chee, Jerry L. Slightom
    Pages 101-119

  13. Agrobacterium-Mediated Transformation of Potato Genotypes

    • Amar Kumar
    Pages 121-128

  14. Agrobacterium-Mediated Transformation of Soft Fruit Rubus, Ribes, and Fragaria

    • Julie Graham, Ronnie J. McNicol, Amar Kumar
    Pages 129-133

  15. High-Frequency and Efficient Agrobacterium-Mediated Transformation ofArabidopsis thaliana Ecotypes “C24” and “Landsberg erecta” Using Agrobacterium tumefaciens

    • Mehdi Barghchi
    Pages 135-147

  16. Transformation Protocols for Broadleaved Trees

    • Trevor M. Fenning, Kevan M. A. Gartland
    Pages 149-165

  17. Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids

    • Michael R. Davey, Rajendra S. Patil, Kenneth C. Lowe, John B. Power
    Pages 167-183

  18. Histochemical GUS Analysis

    • Stanislav Vitha, Karel Beneš, Julian P. Phillips, Kevan M. A. Gartland
    Pages 185-193

  19. Fluorometric GUS Analysis for Transformed Plant Material

    • Kevan M. A. Gartland, Julian P. Phillips, Stanislav Vitha, Karel Beneš
    Pages 195-199

  20. The Detection of Neomycin Phosphotransferase Activity in Plant Extracts

    • Ortrun Mittelsten Scheid, Gabriele Neuhaus-Url
    Pages 201-206
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Keywords

About this book

Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants.

Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.

Editors and Affiliations

  • Department of Molecular and Life Sciences, University of Abertay Dundee, Scotland, UK

    Kevan M. A. Gartland

  • Department of Life Science, Plant Genetic Manipulation Group, University of Nottingham, UK

    Michael R. Davey

Bibliographic Information

  • Book Title: Agrobacterium Protocols

  • Editors: Kevan M. A. Gartland, Michael R. Davey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033023

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 417

  • Topics: Cell Biology

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