Overview
- Editors:
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Christopher Jones
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National Institute for Biological Standards and Control, Potters Bar, UK
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Barbara Mulloy
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National Institute for Biological Standards and Control, Potters Bar, UK
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Mark R. Sanderson
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Department of Biophysics, King’s College, London, UK
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Table of contents (14 protocols)
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- Jane V. Skelly, C. Bernadette Madden
Pages 23-53
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- Sherin S. Abdel-Meguid, David Jeruzalmi, Mark R. Sanderson
Pages 55-86
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- Ian J. Tickle, Huub P. C. Driessen
Pages 173-203
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- Alberto D. Podjarny, Bernard Rees, Alexandre G. Urzhumtsev
Pages 205-226
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- Eric Westhof, Philippe Dumas
Pages 227-244
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- David G. Brown, Paul S. Freemont
Pages 293-318
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- Elizabeth Fry, Derek Logan, David Stuart
Pages 319-363
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Back Matter
Pages 389-394
About this book
The volumes in the series, Methods in Molecular Biology, are conceived with the biochemist and molecular biologist in mind. The present book, Crystallographic Methods andProtocols, concentrates on the use of X-ray crystallography to solve the detailed three dimensional structuresofproteins, nucleic acids, andtheir complexes. Such a structure determination is a major undertaking, demands expertise in a range of skills, and requires considerable resources. The biologically trained worker will probably first become involved when identifying an important scientific problem whose solution would benefit from a full structure. The protein or nucleic acid at issue must be sequenced and prepared to high purity in appropriate quantities, probably by either chemical synthesis for nucleic acids or genetic engineering for proteins. CrystallographicMethods andPro tocols aims to give biologically trained workers an insight into the techniques used to crystallize their proteins, obtain the raw X-ray data, and solve and refine the structure. The aim ofa crystal structure determination is to provide infor mation that will solve biologically relevant problems; that process normally requires a high resolution structure. The preparationofsuit able crystals for a high resolution structure remains the major bottle neck in structure determination, and the effective application of appropriate genetic engineering and biochemical techniques in the initial stages pays ahandsome dividend later. The productionofsuit able proteins by recombinant methods and the preparationofcrystal line derivatives are covered by Skelly and Madden in Chapter 2. Before beginning the data collection required for a high resolution structure, a preliminary characterizationofthe crystals is carried out.
Reviews
...fills an important niche...delivers what it promises: methods and protocols; very basic and down-to-earth, but all the more useful...I warmly recommend this book to newcomers to the field as a hands-on guide to many of the varied activities a macromolecular crystallographer faces.-FEBS Letters
