Neurotrophic Factors

1. Front Matter

   Pages i-xvii

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2. Purification and Microsequencing of Neurotrophic Proteins

   • W. H. Fischer, D. Schubert

   Pages 1-23

3. Identification of Neurotrophic Factors by Molecular Biological Techniques

   • Karoly Nikolics

   Pages 25-55

4. Visualization and Quantitation of Neurotrophin mRNAs

   • Hoakan Persson, Patrik Ernfors

   Pages 57-106

5. Molecular Analysis of Neurotrophin Genes

   • Gerhard Heinrich, Stella Martin

   Pages 107-153

6. Production of Recombinant Neurotrophic Factors

   • Louis E. Burton

   Pages 155-175

7. Production of Genetically Engineered Cells Releasing Neurotrophic Factors

   • Jasodhara Ray, Michael D. Kawqja, Fred H. Gage

   Pages 177-211

8. Characterization and Localization of Receptors for Neurotrophic Factors

   • P. M. Richardson, V. M. K. Verge, R. J Riopelle

   Pages 213-229

9. Transgenic Expression of Neurotrophic Factors and Their Receptors

   • L. J. Huber, M. V Chao

   Pages 231-245

10. Retrograde Axonal Transport

    • Vassilis E. Koliatsos, Donald L. Price

    Pages 247-290

11. Experimental Systems to Study Neurotrophic Factor Effects on Rat Brain Cells

    • Franz Hefti, Dalia Araujo, Klaus D. Beck, Beat Knusel, Paul A. Lapchak, Patrick P. Michel et al.

    Pages 291-329

12. Nonhuman Primate Models in Trophic Factor Research

    • Vassilis E. Koliatsos, Donald L. Price

    Pages 331-370

13. Models to Evaluate Effects of Neurotrophic Factors on Axonal Regeneration

    • Siluio Varon, Theo Hagg

    Pages 371-406

14. Back Matter

    Pages 407-422

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The field of neurotrophic factors has witnessed exp- sive growth in the past decade. As is usual in scientific in- vation, this progress has been closely associated with methodological advances. The introduction of molecular b- logical techniques into the neurotrophic factor field led to the discovery of new families of neurotrophic growth f- tors and their receptors. Production of growth factors by recombinant technology played a crucial part. The example of nerve growth factor, the paradigmatic neurotrophic factor, illustrates this point. A decade ago investigators were forced to purify small quantities of this protein from murine salivary glands, but much larger qu- tities of recombinant nerve growth factor are now available for experimentation as well as clinical development. A decade ago there was a controversy about the existence of nerve growth factor in the brain and the immunoassays used for its measurement, but current publications report the precise localization of gene expression for nerve growth factor and its receptor in the brain. Neurotrophic Factors aims at presenting the techniques that have been crucial to the realization of these rapid advances and thus have helped propel the neurotrophic factors field to its current status of high visibility. These techniques range from molecular biological methods used for cloning and production, to cell culture methods for assessing biological activities, to animal models of nervous system injury (nec- sary for the development of therapeutic agents from neurotrophic factors).

...strength of the book lies in the comprehensive methodology...a welcome addition to any neurobiologist's library. - Journal of Neurochemistry

• University of Saskatchewan, Saskatoon, Edmonton, Canada, Canada

  Alan A. Boulton, Glen B. Baker

• University of Southern California, Los Angeles

  Franz Hefti

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