PCR Protocols

1. Front Matter

   Pages i-xiv

   PDF

2. Polymerase Chain Reaction

   • Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White

   Pages 1-29

3. Selection of Primers for Polymerase Chain Reaction

   • Wojciech Rychlik

   Pages 31-40

4. Direct Radioactive Labeling of Polymerase Chain Reaction Products

   • Tim McDaniel, Stephen J. Meltzer

   Pages 41-43

5. Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays

   • Puja Agarwal, Bruce A. White

   Pages 45-50

6. Nonradioactive Labeling of Polymerase Chain Reaction Products

   • Udo Reischl, Rüdiger Rüger, Christoph Kessler

   Pages 51-62

7. Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography

   • Elena D. Katz

   Pages 63-73

8. Use of Polymerase Chain Reaction for Screening Transgenic Mice

   • Shizhong Chen, Glen A. Evans

   Pages 75-80

9. Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue

   • Matthias Volkenandt, Adam P. Dicker, Renato Fanin, Debabrata Banerjee, Anthony Albino, Joseph R. Bertino

   Pages 81-88

10. The Use of Polymerase Chain Reaction for Chromosome Assignment

    • Michael Jaye, Craig A. Dionne

    Pages 89-94

11. Mapping MHC Class II Genes and Disease-Susceptibility

    • Stephen C. Bain, John A. Todd

    Pages 95-112

12. The Use of the Polymerase Chain Reaction and the Detection of Amplified Products

    • Robert C. Allen, Bruce Budowle

    Pages 113-128

13. Determination of Loss of Heterozygosity Using Polymerase Chain Reaction

    • Stephen J. Meltzer

    Pages 129-136

14. Direct Sequencing of Polymerase Chain Reaction Products

    • Stephen J. Meltzer

    Pages 137-141

15. Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction

    • Adam P. Dicker, Matthias Volkenandt, Joseph R. Bertino

    Pages 143-152

16. Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction

    • Gerd P. Pfeifer, Arthur D. Riggs

    Pages 153-168

17. RNA Template-Specific Polymerase Chain Reaction (RS-PCR)

    • Alan R. Shuldiner, Riccardo Perfetti, Jesse Roth

    Pages 169-176

18. Quantitative Measurement of Relative Gene Expression in Human Tumors

    • Tetsuro Horikoshi, Kathleen Danenberg, Matthias Volkenandt, Thomas Stadlbauer, Peter V. Danenberg

    Pages 177-188

19. Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification

    • Cheng-Ming Chiang, Louise T. Chow, Thomas R. Broker

    Pages 189-198

20. Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression

    • Alice L. Witsell, Lawrence B. Schook

    Pages 199-204

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

...affords many new insights and contributions of the first order...an excellent book of the highest interest.-Cellular and Molecular Biology

• University of Connecticut Health Center, Farmington

  Bruce A. White

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