Protocols in Molecular Parasitology

1. Front Matter

   Pages i-xiv

   PDF

2. Culturing and Biological Cloning of Trypanosoma brucei

   • Mark Carrington

   Pages 1-13

3. Culturing and Biological Cloning of Trypanosoma cruzi

   • Michael A. Miles

   Pages 15-28

4. In Vitro Cultivation and Biological Cloning of Leishmania

   • David A. van Es

   Pages 29-41

5. Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages)

   • Martin Read, John E. Hyde

   Pages 43-55

6. Synchronization and Cloning of Malaria Parasites

   • David Walliker, Geoffrey Beale

   Pages 57-66

7. The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies

   • Richard Carter, Lisa Ranford-Cartwright, Pietro Alano

   Pages 67-88

8. The Culture of Schistosoma mansoni and Production of Life Cycle Stages

   • Fiona Hackett

   Pages 89-99

9. Preparation of DNA and RNA from Trypanosoma brucei

   • Mark Carrington

   Pages 101-111

10. Isolation of RNA and DNA from Trypanosoma cruzi

    • Frank Ashall

    Pages 113-122

11. Isolation of DNA and RNA from Leishmania

    • John M. Kelly

    Pages 123-131

12. The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum

    • John E. Hyde, Martin Read

    Pages 133-143

13. Extraction and Purification of Nucleic Acids from Schistosomes

    • Simon A. Jeffs, Andrew J. Simpson

    Pages 145-154

14. Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting

    • Margaret A. Hughes, Julian M. Crampton

    Pages 155-167

15. The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification

    • Margaret A. Hughes, Julian M. Crampton

    Pages 169-189

16. Analysis of the DNA of Parasitic Protozoa by Flow Cytometry

    • James A. Dvorak

    Pages 191-204

17. PCR orLowLevel Detection of Malaria Parasites in Blood

    • Witoon Trasophon, Sakol Panyim

    Pages 205-211

18. PCR Methods for Identification of Point Mutations and Gene Rearrangements

    • David S. Peterson

    Pages 213-222

19. Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques

    • Vikki M. Marshall, Ross L. Coppel

    Pages 223-248

20. Ribosomal RNA Probes for Detection and Identification of Species

    • Jean-Pierre Baehellerie, Liang-Hu Qu

    Pages 249-263

Protocols in Molecular Parasitology offers a wide range of experi­ mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology. This is a field that has expanded enormously in scope and potential over the last decade, although it is as yet significantly underrepresented in the literature out­ side of academic journals. The need for manuals such as this one stems in part from the increasing difficulty of reproducing experimental tech­ niques from reports in standard journal papers, where pressures on space usually result in highly abbreviated descriptions. The "Notes" sections of the following chapters are particularly aimed at counteracting this problem. Since the emphasis of the book is on aspects of molecular analysis, it was possible to devote only a limited amount of space to describing protocols for culture and isolation of the primary parasitic material. Therefore, of the numerous parasitic systems that are currently being investigated in laboratories around the world, only techniques for c- turing the "big five" are described here, since they collectively account for the great bulk of research in this field. It is also possible that some minor front-end modifications to certain protocols may be necessary, depending on exactly which parasite is being studied.

...well-written and thoughtfully assembled...will be welcomed by recent arrivals...and regularly utilized by instructors of future investigators. - Analytical Biochemistry

• University of Manchester Institute of Science and Technology, Manchester, UK

  John E. Hyde

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