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Protocols in Molecular Parasitology

  • Book
  • © 1993

Overview

Editors:
  1. John E. Hyde

    1. University of Manchester Institute of Science and Technology, Manchester, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 21)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Culturing and Biological Cloning of Trypanosoma brucei

    • Mark Carrington
    Pages 1-13

  3. Culturing and Biological Cloning of Trypanosoma cruzi

    • Michael A. Miles
    Pages 15-28

  4. In Vitro Cultivation and Biological Cloning of Leishmania

    • David A. van Es
    Pages 29-41

  5. Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages)

    • Martin Read, John E. Hyde
    Pages 43-55

  6. Synchronization and Cloning of Malaria Parasites

    • David Walliker, Geoffrey Beale
    Pages 57-66

  7. The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies

    • Richard Carter, Lisa Ranford-Cartwright, Pietro Alano
    Pages 67-88

  8. The Culture of Schistosoma mansoni and Production of Life Cycle Stages

    • Fiona Hackett
    Pages 89-99

  9. Preparation of DNA and RNA from Trypanosoma brucei

    • Mark Carrington
    Pages 101-111

  10. Isolation of RNA and DNA from Trypanosoma cruzi

    • Frank Ashall
    Pages 113-122

  11. Isolation of DNA and RNA from Leishmania

    • John M. Kelly
    Pages 123-131

  12. The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum

    • John E. Hyde, Martin Read
    Pages 133-143

  13. Extraction and Purification of Nucleic Acids from Schistosomes

    • Simon A. Jeffs, Andrew J. Simpson
    Pages 145-154

  14. Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting

    • Margaret A. Hughes, Julian M. Crampton
    Pages 155-167

  15. The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification

    • Margaret A. Hughes, Julian M. Crampton
    Pages 169-189

  16. Analysis of the DNA of Parasitic Protozoa by Flow Cytometry

    • James A. Dvorak
    Pages 191-204

  17. PCR orLowLevel Detection of Malaria Parasites in Blood

    • Witoon Trasophon, Sakol Panyim
    Pages 205-211

  18. PCR Methods for Identification of Point Mutations and Gene Rearrangements

    • David S. Peterson
    Pages 213-222

  19. Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques

    • Vikki M. Marshall, Ross L. Coppel
    Pages 223-248

  20. Ribosomal RNA Probes for Detection and Identification of Species

    • Jean-Pierre Baehellerie, Liang-Hu Qu
    Pages 249-263
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About this book

Protocols in Molecular Parasitology offers a wide range of experi­ mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology. This is a field that has expanded enormously in scope and potential over the last decade, although it is as yet significantly underrepresented in the literature out­ side of academic journals. The need for manuals such as this one stems in part from the increasing difficulty of reproducing experimental tech­ niques from reports in standard journal papers, where pressures on space usually result in highly abbreviated descriptions. The "Notes" sections of the following chapters are particularly aimed at counteracting this problem. Since the emphasis of the book is on aspects of molecular analysis, it was possible to devote only a limited amount of space to describing protocols for culture and isolation of the primary parasitic material. Therefore, of the numerous parasitic systems that are currently being investigated in laboratories around the world, only techniques for c- turing the "big five" are described here, since they collectively account for the great bulk of research in this field. It is also possible that some minor front-end modifications to certain protocols may be necessary, depending on exactly which parasite is being studied.

Reviews

...well-written and thoughtfully assembled...will be welcomed by recent arrivals...and regularly utilized by instructors of future investigators. - Analytical Biochemistry

Editors and Affiliations

  • University of Manchester Institute of Science and Technology, Manchester, UK

    John E. Hyde

Bibliographic Information

  • Book Title: Protocols in Molecular Parasitology

  • Editors: John E. Hyde

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032396

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1993

  • Softcover ISBN: 978-0-89603-239-2Published: 11 August 1993

  • eBook ISBN: 978-1-59259-508-2Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 470

  • Topics: Microbiology

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