Enzymatic Analysis

1. Front Matter

   Pages i-x

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2. General Principles

   1. Front Matter

      Pages 2-2

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   2. Pyridine Nucleotides

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 3-21

   3. A Kinetics Primer for the Tissue Analyst

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 23-49

   4. Constriction Pipets

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 51-69

   5. Preparation of Tissues for Analysis

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 71-81

3. Specific Methods and Procedures

   1. Front Matter

      Pages 84-84

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   2. Enzymatic Cycling

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 85-110

   3. A Collection of Metabolite Assays

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 111-228

   4. A Collection of Enzyme Assays

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 229-305

   5. Improvement, Modification, Adaptation, Troubleshooting, and Development of New Methods

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 307-318

4. Quantitative Histochemistry

   1. Front Matter

      Pages 320-320

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   2. Preparation of Tissue and Sections

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 321-328

   3. Dissection and Histological Control

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 329-336

   4. The Quartz Fiber Fishpole Balance

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 337-351

   5. Histochemical Analyses

      • Janet V. Passonneau, Oliver H. Lowry

      Pages 353-362

5. Back Matter

   Pages 365-403

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Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

• enzyme
• enzymes
• NADP
• Nucleotide

...the authors are well recognized for their contributions to histochemistry...For individuals interested in...performing quantitative histochemical methods, this book would be a worthwhile investment.-Modern Pathology

• Washington, USA

  Janet V. Passonneau

• Washington University, St. Louis, USA

  Oliver H. Lowry

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