Spectroscopic Methods and Analyses

1. Front Matter

   Pages i-x

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2. Nmr Spectroscopy

   1. Introduction to Nuclear Magnetic Resonance

      • Christopher Jones, Barbara Mulloy

      Pages 1-14

   2. Structural Studies of Proteins in Solution Using Proton Nuclear Magnetic Resonance

      • David Neuhaus, Philip A. Evans

      Pages 15-67

   3. Peptide Structure Determination by NMR

      • Michael P. Williamson

      Pages 69-85

   4. High-Resolution NMR of DNA and Drug-DNA Interactions

      • Jill Barber, Helen F. Cross, John A. Parkinson

      Pages 87-113

   5. Structural Characterization of the Carbohydrate Moieties of Glycoproteins by High-Resolution 1H-NMR Spectroscopy

      • Herman van Halbeek

      Pages 115-148

   6. The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides

      • Christopher Jones, Barbara Mulloy

      Pages 149-167

   7. Dynamic and Exchange Processes in Macromolecules Studied by NMR Spectroscopy

      • Lu-Yun Lian

      Pages 169-189

3. Nmr Spectroscopy

   1. Introduction to Mass Spectrometry

      • Robin Wait

      Pages 191-213

   2. Laser Desorption Ionization Mass Spectrometry of Bioorganic Molecules

      • Michael Karas, Ute Bahr

      Pages 215-228

   3. 252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins

      • Peter Roepstorff

      Pages 229-235

   4. Fast Atom Bombardment Mass Spectrometry of Peptides

      • Robin Wait

      Pages 237-283

   5. Tandem Mass Spectrometry

      • Catherine E. Costello

      Pages 285-313

4. Nmr Spectroscopy

   1. Mössbauer Spectroscopy

      • Dominic P. E. Dickson

      Pages 315-325

   2. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins

      • Richard Cammack

      Pages 327-344

   3. Resonance Raman Spectroscopy of Metalloproteins Using CW Laser Excitation

      • Roman S. Czernusxewicx

      Pages 345-374

   4. The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins

      • C. David Garner

      Pages 375-390

5. Back Matter

   Pages 391-395

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The three volumes in Methods in Molecular Biology covering Physical Methods of Analysis (vol. 1, Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques; vol. 2, Optical Spectroscopy and Macroscopic Techniques; vol. 3, Cryst- lographic Methods and Techniques) differ from others in this series in several ways. Each volume covers a group of techniques for the char- terization of biological molecules and their interactions that involve the application of modern techniques of physical chemistry. These techniques by and large do not lend themselves to the "hands-on" approach and cannot usually be carried out by the molecular biologist alone, but most often require collaboration with a specialist. The biologist or biochemist contemplating such a collaboration may feel somewhat at a distance from the experimental work and further isolated by the use of the jargons of analytical and physical chemistry. Physical methods have been used in molecular biology from the earliest days, from simple applications of optical spectroscopy to the complexity of X-ray crystallography, and the full range of these me- ods will be covered over the three volumes. The methods dealt with in this first volume have largely developed from beginnings in small molecule chemistry to the point where they play a valuable role in the characterization of biological macromolecules.

The main strength of this volume...is the extensive attention paid to the pragmatic and mundane issues that are only gleaned with difficulty from the literature, yet are absolutely critical for success. Those contemplating initiating studies employing these physical methods would be well served by consulting this manual. - Modern Pathology

• National Institute for Biological Standards and Control, Potters Bar, UK

  Christopher Jones, Barbara Mulloy, Adrian H. Thomas

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