Overview
- Editors:
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Alan A. Boulton
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University of Saskatchewan, Saskatoon, Canada
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Glen B. Baker
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University of Alberta, Edmonton, Canada
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Wolfgang Walz
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University of Saskatchewan, Saskatoon, Canada
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Table of contents (11 protocols)
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- Diane E. Harold, Wolfgang Walz
Pages 1-20
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- Marston Manthorpe, David Muir, Brigitte Pettmann, Silvio Varon
Pages 87-137
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- B. Rogister, J. M. Rigo, P.P. Lefebvre, P. Leprince, P. Delree, D. Martin et al.
Pages 173-200
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- Jeffrey R. Buchhalter, Marc A. Dichter
Pages 241-268
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- Bernhard H. J. Juurlink, Leif Hertz
Pages 269-321
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- Jean de Vellis, Araceli Espinosa de los Monteros
Pages 323-352
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Back Matter
Pages 379-392
About this book
Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.
Reviews
...a great deal of useful and well-referenced basic information, as well as...methods for a large number of different techniques ...with extensive protocols for specific techniques.-Trends in Neurosciences
