Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Gene Transfer and Expression Protocols

  • Book
  • © 1991

Overview

Editors:
  1. E. J. Murray

    1. Roche Products Ltd., Welwyn Garden City, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 7)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (32 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer

    • Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson
    Pages 3-13

  3. Calcium Phosphate Mediated Gene Transfer into Established Cell Lines

    • Hiroto Okayama, Claudia Chen
    Pages 15-21

  4. Transfection of the ChloramphenicolAcetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran

    • Richard A. Lake, Michael J. Owen
    Pages 23-33

  5. Polybrene/DMSOAssisted Gene Transfer

    • Remy Aubin, Michael Weinfeld, Malcolm C. Paterson
    Pages 35-43

  6. Electroporation Technique of DNA Transfection

    • Sarah C. Spencer
    Pages 45-52

  7. Irradiation and Fusion Gene Transfer

    • Peter N. Goodfellow
    Pages 53-61

  8. Direct Use of λ Phage Particles for DNA Transfection

    • Masahiro Ishiura
    Pages 63-80

  9. Cationic Liposome-Mediated Transfection with Lipofectinâ„¢ Reagent

    • Philip L. Felgner
    Pages 81-89

  10. Preparation of High-Molecular-Weight DNA for Use in DNA Transfection

    • Michael A. Tainsky
    Pages 91-98

  11. Chromosome-Mediated Gene Thnsfer

    • Janet M. Shipley, Denise Sheer
    Pages 99-106

  12. Manipulation of Adenovirus Vectors

    • Frank L. Graham, Ludvik Prevec
    Pages 109-128

  13. Manipulation of Vaccinia Virus Vectors

    • Michael Mackett
    Pages 129-146

  14. Manipulation of Baculovirus Vectors

    • Murk J. Bailey, Robert D. Possee
    Pages 147-168

  15. Manipulation of SV40 Vectors

    • Marie-Louise Hammurskjöld
    Pages 169-180

  16. Choice and Manipulation of Retroviral Vectors

    • Jay P. Morgenstern, Hartmut Land
    Pages 181-206

  17. Use of Tissue-Plasminogen Activator as a Reporter Gene

    • Nicholas C. Wrighton
    Pages 209-215

  18. Use of Escherichiu coli (E. coli) lacZ (β-Galactosidase) as a Reporter Gene

    • Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg
    Pages 217-235

  19. Application of the Firefly Luciferase Reporter Gene

    • Vincent Giguère
    Pages 237-241

  20. Use of Vectors to Confer Resistance to Antibiotics G418 and Hygromycin in Stably Transfected Cell Lines

    • Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell
    Pages 245-256
Back to top

About this book

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .

Reviews

Popular....Friendly guide...follows the established format...combining recipes and protocols with background information and useful troubleshooting hints....Gene Transfer and Expression Protocols is pitched at just the right level and is a worthy addition....A worthwhile and accurate manual written by the right people. -Society for General Microbiology Quarterly

Editors and Affiliations

  • Roche Products Ltd., Welwyn Garden City, UK

    E. J. Murray

Bibliographic Information

  • Book Title: Gene Transfer and Expression Protocols

  • Editors: E. J. Murray

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896031780

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1991

  • Hardcover ISBN: 978-0-89603-178-4Published: 22 April 1991

  • Softcover ISBN: 978-1-4899-4403-0Published: 30 September 2013

  • eBook ISBN: 978-1-59259-494-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 439

  • Number of Illustrations: 33 b/w illustrations

  • Topics: Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation