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Birkhäuser

Protein Analysis and Purification

Benchtop Techniques

  • Book
  • © 2005

Overview

Authors:
  1. Ian M. Rosenberg

    1. G.I. Unit, Massachusetts General Hospital, Boston, USA

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  • Each chapter contains a wealth of practical information, including troubleshooting tips, safety icons and references
  • More than 180 protocols included with materials lists, step-by-step instructions and valuable comments on practical issues such as time considerations and safety issues
  • Includes supplementary material: sn.pub/extras

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xxvi
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  2. An Overview of This Manual

    Pages 1-7

  3. Protein Structure

    Pages 8-23

  4. Tracking the Target Protein

    Pages 24-62

  5. Electrophoretic Techniques

    Pages 63-117

  6. Getting Started with Protein Purification

    Pages 118-152

  7. Membrane Proteins

    Pages 153-176

  8. Transfer and Detection of Proteins on Membrane Supports

    Pages 177-210

  9. Identification of the Target Protein

    Pages 211-258

  10. Identifying and Analyzing Posttranslational Modifications

    Pages 259-323

  11. Chromatography

    Pages 324-384

  12. Recombinant Protein Techniques

    Pages 385-430

  13. Back Matter

    Pages 431-520
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Keywords

About this book

How one goes about analyzing proteins is a constantly evolving ?eld that is no longer solely the domain of the protein biochemist. Inves- gators from diverse disciplines ?nd themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are ?lled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to ?t individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scienti?c research is performed. Bioinformatics and In silico science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.

Authors and Affiliations

  • G.I. Unit, Massachusetts General Hospital, Boston, USA

    Ian M. Rosenberg

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