Sample Preparation Handbook for Transmission Electron Microscopy

1. Front Matter

   Pages i-xxiii

   PDF

2. Methodology: General Introduction

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 1-2

3. Introduction to Materials

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 3-31

4. The Different Observation Modes in Electron Microscopy (SEM, TEM, STEM)

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 33-55

5. Materials Problems and Approaches for TEM and TEM/STEM Analyses

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 57-81

6. Physical and Chemical Mechanisms of Preparation Techniques

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 83-123

7. Artifacts in Transmission Electron Microscopy

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 125-170

8. Selection of Preparation Techniques Based on Material Problems and TEM Analyses

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 171-197

9. Comparisons of Techniques

   • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

   Pages 199-234

10. Conclusion: What Is a Good Sample?

    • Jeanne Ayache, Luc Beaunier, Jacqueline Boumendil, Gabrielle Ehret, Danièle Laub

    Pages 235-236

11. Back Matter

    Pages 237-250

    PDF

Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.

• Sample preparation technique for TEM
• Structure analysis
• biology
• chemical analysis
• electron microscopy
• materials science
• microscopy
• sample preparation physics
• spectroscopic analysis
• thin slices protocols
• transmission electron microscopy
• Biological Microscopy

• Laboratoire de Microscopie Moléculaire et Cellulaire, Institut Gustave Roussy Unité mixte CNRS-UMR8126-IGR, Villejuif CX, France

  Jeanne Ayache

• Labo. Interfaces et Systèmes Electrochimiques, Université Paris VI UPR 15 CNRS Boîte courrier 133, Paris CX 05, France

  Luc Beaunier

• Centre de Microscopie Electronique Appliquée à la Biologie et à la Géologie, Université Lyon I, Villeurbanne CX, France

  Jacqueline Boumendil

• Inst. Physique et Chimie des Matériaux, Université Strasbourg CNRS-UMR 7504, Strasbourg CX 2, France

  Gabrielle Ehret

• Faculté des Sciences de Base Centre Interdisciplinaire de Microscopie Electronique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

  Danièle Laub

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