Cellular and Molecular Methods in Neuroscience Research

1. Front Matter

   Pages I-XIV

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2. Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells

   • Elena Cattaneo, Luciano Conti

   Pages 1-13

3. Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors

   • Alain Beaudet, Alexander C. Jackson, Franck Vandenbulcke

   Pages 15-28

4. Transfection Methods for Neurons in Primary Culture

   • Christoph Kaether, Martin KÖhrmann, Carlos G. Dotti, Francesca Ruberti

   Pages 29-36

5. Polyethylenimine: A Versatile Cationic Polymer for Plasmid-Based Gene Delivery in the CNS

   • Barbara A. Demeneix, Gregory F. Lemkine, Hajer Guissouma

   Pages 37-51

6. Transfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK 293 Cells

   • Stefano Vicini, Jin Hong Li, Wei Jian Zhu, Karl Krueger, Jian Feng Wang

   Pages 53-66

7. Neuronal Transfection Using Particle-Mediated Gene Transfer

   • Harold Gainer, Raymond L. Fields, Shirley B. House

   Pages 67-84

8. Analysis of Gene Expression in Genetically Labeled Single Cells

   • Stefano Gustincich, Andreas Feigenspan, Elio Raviola

   Pages 85-108

9. Immunocytochemistry and In Situ Hybridization: Their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons

   • Marc Landry, André Calas

   Pages 119-143

10. In Situ Reverse Transcription PCR for Detection of mRNA in the CNS

    • Helle Broholm, Steen Gammeltoft

    Pages 145-159

11. Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity

    • Patrizia Aimar, Laura Lossi, Adalberto Merighi

    Pages 161-180

12. Combined Electrophysiological and Morphological Analyses of CNS Neurons

    • Alfredo Ribeiro-da-Silva, Yves De Koninck

    Pages 181-201

13. Tract Tracing Methods at the Light Microscopic Level

    • Marina Bentivoglio, Giuseppe Bertini

    Pages 203-219

14. Tract Tracing Methods at the Ultrastructural Level

    • Isaura Tavares, Armando Almeida, Deolinda Lima

    Pages 221-234

15. In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS

    • Laura Lossi, Silvia Mioletti, Patrizia Aimar, Renato Bruno, Adalberto Merighi

    Pages 235-258

16. Confocal Imaging of Nerve Cells and Their Connections

    • Andrew J. Todd

    Pages 259-272

17. Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices

    • Wim Scheenen, Giorgio Carmignoto

    Pages 273-283

18. Back Matter

    Pages 285-303

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Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well- known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal.

• Ethylen
• Vivo
• cell biology
• gene transfer
• neurobiology
• neurons
• neuropeptides
• neuroscience
• tissue

• Department of Veterinary Morphophysiology Rita Levi Montalcini Center Brain Repair, University of Torino, Gruglasco, Italy

  Adalberto Merighi

• Department of Experimental and Biomedical Sciences CNR Center for the Study of Biomembranes, University of Padova, Padova, Italy

  Giorgio Carmignoto

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