Microarrays

1. Front Matter

   Pages i-xv

   PDF

2. Overview and New Detection Method

   1. The Current Status of DNA Microarrays

      • Leming Shi, Roger G. Perkins, Weida Tong

      Pages 3-24

   2. Electrochemical Detection on Microarrays

      • Kilian Dill, Andrey Ghindilis

      Pages 25-34

3. Fluidic Manipulation and Microarrays

   1. Fully Integrated Microfluidic Device for Direct Sample-to-Answer Genetic Analysis

      • Robin H. Liu, Piotr Grodzinski

      Pages 37-65

   2. Integrated Microfluidic Devices for Automated Microarray-Based Gene Expression and Genotyping Analysis

      • Robin H. Liu, Mike Lodes, H. Sho Fuji, David Danley, Andrew McShea

      Pages 67-93

4. Statistical Data Evaluation on Microarrays

   1. Intensity Concentration Relationships for Electrochemical Detection

      • Mervyn Thomas

      Pages 97-117

5. Applications

   1. Genotyping Arrays

      • Michael J. Lodes, Dominic Suciu, David Danley, Andrew McShea

      Pages 121-138

   2. Peptide-Based Microarray

      • Resmi C. Panicker, Hongyan Sun, Grace Y. J. Chen, Shao Q. Yao

      Pages 139-167

   3. Protein Microarrays for the Detection of Biothreats

      • Amy E. Herr

      Pages 169-190

   4. Photo-Generation of Carbohydrate Microarrays

      • Gregory T. Carroll, Denong Wang, Nicholas J. Turro, Jeffrey T. Koberstein

      Pages 191-210

   5. Expression Profiling Using Microfluidic Living Cell Arrays

      • Kevin R. King, Martin L. Yarmush, Arul Jayaraman

      Pages 211-226

   6. New Approaches to the Synthesis of Addressable Microarray Molecular Libraries

      • Karl Maurer, Kevin D. Moeller

      Pages 227-246

   7. eSensor®A Microarray Technology Based on Electrochemical Detection of Nucleic Acids and Its Application to Cystic Fibrosis Carrier Screening

      • Michael R. Reed, William A. Coty

      Pages 247-260

6. Future Improvements in Microarray Sensing

   1. Use of Redox Enzymes for the Electrochemical Detection of Sequence-Specific DNA and Immunochemical Entities

      • Kilian Dill, Andrey Ghindilis

      Pages 263-270

   2. Biochip Platforms for DNA Diagnostics

      • Anil K. Deisingh, Adilah Guiseppi-Wilson, Anthony Guiseppi-Elie

      Pages 271-297

   3. MagArray Biochips for Protein and DNA Detection with Magnetic Nanotags: Design, Experiment, and Signal-to-Noise Ratio

      • Sebastian J. Osterfeld, Shan X. Wang

      Pages 299-314

   4. Bar Coding Platforms for Nucleic Acid and Protein Detection

      • Uwe R. Müller

      Pages 315-338

   5. Electrochemical Nanoparticle-Based Sensors

      • Joseph Wang

      Pages 339-353

7. Back Matter

   Pages 355-356

   PDF

Combinatorial chemistry is used to find materials that form sensor microarrays. This book discusses the fundamentals, and then proceeds to the many applications of microarrays, from measuring gene expression (DNA microarrays) to protein-protein interactions, peptide chemistry, carbodhydrate chemistry, electrochemical detection, and microfluidics.

• Biosensor
• DNA
• Expression
• Microarray
• PCR
• biochip
• chemistry
• enzymes
• gene expression
• genes
• hybridization
• nanoparticle
• preparation
• protein
• sensors
• biochemical engineering

From the reviews:

"Microarray technology is not without its challenges and limitations … this book highlight novel approaches that may address some of those issues and offer suggestions of new strategies on the horizon. … I found many of the topics in the book to be interesting … . it has a considerable amount of useful material for someone who … is looking for a broader perspective on applications. It will also be useful to those focused on electrochemical detection and microfluidics in microarrays." (Peter Wentzell, Journal of the American Chemical Society, Vol. 131 (36), 2009)

• CombiMatrix Corporation, Inc. 6500 Harbour Heights Parkway, Mukilteo, USA

  Kilian Dill

• Osmetech Molecular Diagnostics, Pasadena, USA

  Robin Hui Liu

• National Institutes of Health National Cancer Institute, Bethesda, USA

  Piotr Grodzinski

Policies and ethics