Genetic Engineering

1. Front Matter

   Pages i-xviii

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2. Evolution of Transport Proteins

   • Milton H. Saier Jr.

   Pages 1-10

3. Mechanisms of Apoptosis Repression

   • Collin C. Q. Vu, John A. Cidlowski

   Pages 11-33

4. Cytokine Activation of Transcription

   • Kerri A. Mowen, Michael David

   Pages 35-44

5. Enzymatic Approaches to Glycoprotein Synthesis

   • Pamela Sears, Thomas Tolbert, Chi-Huey Wong

   Pages 45-68

6. Vector Design and Development of Host Systems for Pseudomonas

   • Herbert P. Schweizer, Tung T. Hoang, Katie L. Propst, Henry R. Ornelas, RoxAnn R. Karkhoff-Schweizer

   Pages 69-81

7. Genetic and Biochemical Studies on the Assembly of an Enveloped Virus

   • Timothy L. Tellinghuisen, Rushika Perera, Richard J. Kuhn

   Pages 83-112

8. Enzyme and Pathway Engineering for Suicide Gene Therapy

   • Margaret E. Black

   Pages 113-127

9. Deconstructing a Conserved Protein Family: The Role of MCM Proteins in Eukaryotic DNA Replication

   • Sally G. Pasion, Susan L. Forsburg

   Pages 129-155

10. Expression of Foreign Genes in the yeast Pichia pastoris

    • Geoffrey P. Lin Cereghino, Anthony J. Sunga, Joan Lin Cereghino, James M. Cregg

    Pages 157-169

11. Protein Splicing and its Applications

    • Izabela Giriat, Thomas W. Muir, Francine B. Perler

    Pages 171-199

12. Global Transcript Expression Profiling by Serial Analysis of Gene Expression (SAGE)

    • Hamish S. Scott, Roman Chrast

    Pages 201-219

13. Back Matter

    Pages 221-231

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Over the past decade, our laboratory and others have been concerned with molecular archaeological studies aimed at revealing the origins and evolutionary histories of permeases (1). These studies have revealed that several different families, defined on the basis of sequence similarities, arose independently of each other, at different times in evolutionary history, following different routes. When complete microbial genomes first became available for analysis, we adapted p- existing software and designed new programs that allowed us quickly to identify probable transmembrane proteins, estimate their topologies and determine the likelihood that they function in transport (2). This work allowed us to expand previously-recognized families and to identify dozens of new families. All of this work then led us to attempt to design a rational but comprehensive classification system that would be applicable to the complete complement of transport systems found in all living organisms (3). The classification system that we have devised is based primarily on mode of transport and energy coupling mechanism, secondarily on molecular phylogeny, and lastly on the substrate specificities of the individual permeases (4).

• Bur
• DNA
• biochemistry
• enzymes
• evolution
• gene expression
• gene therapy
• genes
• genetic engineering
• genetics
• transcription

• Brookhaven National Laboratory, Upton

  Jane K. Setlow

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

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