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Introduction to Cell and Tissue Culture

Theory and Technique

  • Book
  • © 1998

Overview

Authors:
  1. Jennie P. Mather

    1. Genentech Inc., South San Francisco

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    You can also search for this author in PubMed   Google Scholar

  2. Penelope E. Roberts

    1. Genentech Inc., South San Francisco

    View author publications

    You can also search for this author in PubMed   Google Scholar

Part of the book series: Introductory Cell and Molecular Biology Techniques (ICMB)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xv
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  2. Introduction

    Pages 1-8

  3. Setting Up a Cell Culture Laboratory

    Pages 9-23

  4. The Physical Environment

    Pages 25-40

  5. Media

    Pages 41-62

  6. Standard Cell Culture Techniques

    Pages 63-87

  7. Looking at Cells

    Pages 89-115

  8. Contamination: How to Avoid It, Recognize It, and Get Rid of It

    Pages 117-127

  9. Serum-Free Culture

    Pages 129-150

  10. Primary Cultures

    Pages 151-163

  11. Establishing a Cell Line

    Pages 165-174

  12. Special Growth Conditions

    Pages 175-194

  13. Cell Culture for Commercial Settings

    Pages 195-204

  14. Back Matter

    Pages 205-241
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Keywords

About this book

It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The­ ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought­ ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method­ ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for­ mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use­ ful sources of information. In addition to the fundamentals, attention is also given to mod­ ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot­ ed to any of the many disciplines to which cell culture methodology is applicable.

Authors and Affiliations

  • Genentech Inc., South San Francisco

    Jennie P. Mather, Penelope E. Roberts

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