Protein Engineering Techniques

1. Front Matter

   Pages i-xiv

   PDF

2. World of Proteins: Structure-Function Relationships and Engineering Techniques

   • Krishna Mohan Poluri, Khushboo Gulati

   Pages 1-25

3. Expanding the Synthetic Protein Universe by Guided Evolutionary Concepts

   • Krishna Mohan Poluri, Khushboo Gulati

   Pages 27-59

4. Rational Designing of Novel Proteins Through Computational Approaches

   • Krishna Mohan Poluri, Khushboo Gulati

   Pages 61-83

5. Generating the Fancy Protein Basket with De Novo and Combinatorial Approaches

   • Krishna Mohan Poluri, Khushboo Gulati

   Pages 85-102

6. Biotechnological and Biomedical Applications of Protein Engineering Methods

   • Krishna Mohan Poluri, Khushboo Gulati

   Pages 103-134

This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

• Protein Structure-function Relationship
• Computational Approaches to Protein Design
• Synthetic/Engineered Proteins
• Protein Carbohydrate Interaction
• Protein Evolution
• Structure Based Drug Design
• Protein Folding
• Structural Virology
• Therapeutic Proteins
• Protein Drugs

• Department of Biotechnology and Center for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, India

  Krishna Mohan Poluri

• Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India

  Khushboo Gulati

Dr. Krishna Mohan Poluri is currently working as an Asst. Professor at the Department of Biotechnology, IIT-Roorkee. He completed his PhD in Chemistry from the Tata Institute of Fundamental Research TIFR-Mumbai, and Post-doc from Rutgers University. Dr. Poluri worked as a Research scientist at the University of Texas Medical Branch (UTMB-Texas). His areas of expertise are Protein Engineering, Bio-molecular Spectroscopy, Structural Biology, Molecular Biophysics, Bio-molecular Interactions, Glycoimmunology, Structural virology and Structure based design of Therapeutics and Scaffolds. He is an editorial member and reviewer for several journals. He has won several awards and fellowships for his research work. Most prominent being: Young scientist awards from Indian Science Congress association (ISCA) -2009, National Academy of Sciences India (NASI)-2014, and Innovative Young Biotechnologist Award (IYBA)-2013 by DBT.
Ms. Khushboo Gulati is currently pursuing her PhD at Department ofBiotechnology, IIT- Roorkee. She completed her Bachelors in Bioinformatics and Masters in Biotechnology from Punjab University, India. Her Research interests are in the area of Protein Engineering, Molecular Biophysics, Structural Biology and Glycoimmunology including Protein Carbohydrate interactions.

Policies and ethics