World Crop Series

Membrane Receptors

Methods for Purification and Characterization

Editors: Cuatrecasas, Pedro (Ed.)

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About this book

Hardly a decade ago, membrane receptors were an attractive but largely unproven concept. Since that time enormous progress has been made, and we are now able to consider receptors much more concretely. Their existence has been established, their binding properties have been determined, and in some cases, they have been highly purified and their physical-chemical properties studied. It is now even possible to visualize microscopically some receptors. This progress has resulted largely from the development of highly powerful methods. These methods are the subject of this volume. Although considerably diverse, different receptors share certain common pro­ perties, and common problems are encountered in their study. Consequently, a small number of techniques are particularly useful in studying different types of receptors. Thus, it makes sense to speak about membrane receptor methodology. A very apparent problem in the study of membrane receptors is their presence in exceedingly small quantities and in a highly impure state. Therefore, very sensitive and specific techniques are required for their detection, characterization and purification. Such sensitivity and specificity is provided by the ability of receptors to bind certain ligands with very high affmity, and it is not surprising that most of the methods described in this volume depend upon this high affinity binding. The antigen-antibody interaction is of comparable sensitivity and speci­ ficity. Recently, a number of anti-receptor antibodies have been produced or found to occur spontaneously in auto-immune diseases. Undoubtedly, more will be produced in the future.

Table of contents (8 chapters)

  • Receptor Binding Assays

    Hollenberg, Morley D. (et al.)

    Pages 1-31

  • Solubilization and Characterization of Membrane Proteins

    Reynolds, Jacqueline A.

    Pages 33-59

  • Affinity Chromatography for Membrane Receptor Purification

    Jacobs, Steven (et al.)

    Pages 61-86

  • Affinity Labeling of Hormone Receptors and Other Ligand Binding Proteins

    Linsley, Peter S. (et al.)

    Pages 87-113

  • Immunochemical Identification and Characterization of Membrane Receptors

    Bjerrum, O. J. (et al.)

    Pages 115-156

Buy this book

eBook $74.99
price for USA (gross)
  • ISBN 978-94-009-5866-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.00
price for USA
  • ISBN 978-94-009-5868-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Receptors
Book Subtitle
Methods for Purification and Characterization
Editors
  • Pedro Cuatrecasas
Series Title
World Crop Series
Copyright
1981
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media Dordrecht
eBook ISBN
978-94-009-5866-1
DOI
10.1007/978-94-009-5866-1
Softcover ISBN
978-94-009-5868-5
Edition Number
1
Number of Pages
XII, 240
Number of Illustrations and Tables
26 b/w illustrations
Topics