Fundamentals of Fluorescence Microscopy

Exploring Life with Light

Authors: Mondal, Partha Pratim, Diaspro, Alberto

  • A comprehensive text that starts from the fundamental optical imaging concepts
  • Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
  • An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
  • A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
  • Contains three useful Appendices
  • Lists of recommended books at the end of each chapter, to expand on various specialized topics
  • Written by two acknowledged experts in this rapidly evolving, interdisciplinary field
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eBook $54.99
price for USA (gross)
  • ISBN 978-94-007-7545-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $69.99
price for USA
  • ISBN 978-94-007-7544-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $69.99
price for USA
  • ISBN 978-94-017-7966-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Rent the ebook  
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About this Textbook

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications.  The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

About the authors

Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, Univ.of Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at Univ.of Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
Alberto Diaspro is a member of the Bioengineering and  Robotics  PhD Advisory Committee (University of Genova) and  affiliated with the Institute of Biophysics of the National Research Council (CNR). He founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009?2011), President of OWLS (Optics with Life Sciences) (2010?2014) and member of the International Relations Committee of the Biophysical Society. ISI Pubs > 250, H=30, cit > 4000 (Google Scholar). AD is involved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.

Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to ‘Microscopy, Research and Technique (Wiley).

Reviews

“Mondal and Diaspro have produced an introductory text providing all the necessary scientific background for students in applied physics, biophysics and biomedical engineering, Their text is also intended to be used by scientists and engineers in both industry and academia. … The book is generally well written for the intended readership … . It is nicely produced with large (almost A4 sized) glossy pages and some very attractive and interesting coloured images.” (Peter V. E. McClintock, Contemporary Physics, Vol. 58 (2), December, 2016)


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Table of contents (11 chapters)

  • Ray Optics, Wave Optics and Imaging System Design

    Mondal, Partha Pratim (et al.)

    Pages 3-31

  • Basics of Electromagnetic Theory for Fluorescence Microscopy

    Mondal, Partha Pratim (et al.)

    Pages 33-51

  • Electric Field Effects in Optical Microscopy Systems

    Mondal, Partha Pratim (et al.)

    Pages 53-70

  • Quantum Description of Radiation Field and Optical Microscopy

    Mondal, Partha Pratim (et al.)

    Pages 71-94

  • Molecular Physics of Fluorescent Markers

    Mondal, Partha Pratim (et al.)

    Pages 95-107

Buy this book

eBook $54.99
price for USA (gross)
  • ISBN 978-94-007-7545-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $69.99
price for USA
  • ISBN 978-94-007-7544-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $69.99
price for USA
  • ISBN 978-94-017-7966-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Rent the ebook  
  • Rental duration: 1 or 6 month
  • low-cost access
  • online reader with highlighting and note-making option
  • can be used across all devices
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Bibliographic Information

Bibliographic Information
Book Title
Fundamentals of Fluorescence Microscopy
Book Subtitle
Exploring Life with Light
Authors
Copyright
2014
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media Dordrecht
Distribution Rights
Distribution rights for India: Delhi Book Store, New Delhi, India
eBook ISBN
978-94-007-7545-9
DOI
10.1007/978-94-007-7545-9
Hardcover ISBN
978-94-007-7544-2
Softcover ISBN
978-94-017-7966-1
Edition Number
1
Number of Pages
XV, 218
Number of Illustrations and Tables
85 b/w illustrations, 55 illustrations in colour
Topics