Topics in Current Genetics

Site-directed insertion of transgenes

Editors: Renault, Sylvaine, Duchateau, Philippe (Eds.)

  • No other book published in this field of research
  • Presentation of all the innovative methodologies in genome engineering
  • From basic science to practical applications
  • Concerns a broad range of organisms (from bacteria to mammals)
  • From basic science to practical applications
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Buy this book

eBook $189.00
price for USA (gross)
  • ISBN 978-94-007-4531-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $239.00
price for USA
  • ISBN 978-94-007-4530-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $239.00
price for USA
  • ISBN 978-94-017-8075-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.

Table of contents (14 chapters)

  • Transgene Site-Specific Integration: Problems and Solutions

    Bire, Solenne (et al.)

    Pages 3-39

  • Designing Non-viral Targeted Integrating Vectors for Genome Engineering in Vertebrates

    Sinzelle, Ludivine (et al.)

    Pages 41-67

  • Gene Targeting and Homologous Recombination in Saccharomyces cerevisiae

    Miné-Hattab, Judith (et al.)

    Pages 71-89

  • Homologous Recombination in Mammals

    Barascu, Aurélia (et al.)

    Pages 91-120

  • Engineered Zinc Finger Nucleases for Targeted Genome Editing

    Ramirez, Cherie L. (et al.)

    Pages 121-145

Buy this book

eBook $189.00
price for USA (gross)
  • ISBN 978-94-007-4531-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $239.00
price for USA
  • ISBN 978-94-007-4530-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $239.00
price for USA
  • ISBN 978-94-017-8075-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Site-directed insertion of transgenes
Editors
  • Sylvaine Renault
  • Philippe Duchateau
Series Title
Topics in Current Genetics
Series Volume
23
Copyright
2013
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media Dordrecht
eBook ISBN
978-94-007-4531-5
DOI
10.1007/978-94-007-4531-5
Hardcover ISBN
978-94-007-4530-8
Softcover ISBN
978-94-017-8075-9
Series ISSN
1610-2096
Edition Number
1
Number of Pages
XIV, 394
Topics