Cell Engineering

Cell Line Development

Editors: Al-Rubeai, Mohamed (Ed.)

  • Presents an overall picture of the current state of knowledge in Cell Line Development
  • Designed to offer a state-of-the-art review with in–depth assessments and perspectives of expression engineering through understanding the molecular and cellular basis of cell culture systems
  • The various chapters describe the necessary methodologies for using cell engineering tools for the improvement of cell culture processes
  • Describes the most recent methods used to establish mammalian host cell lines and expression systems, optimisation of selection systems to reduce development times and improvement of glycosylation and secretion
see more benefits

Buy this book

eBook $199.00
price for USA (gross)
  • ISBN 978-90-481-2245-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $259.00
price for USA
  • ISBN 978-90-481-2244-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $259.00
price for USA
  • ISBN 978-94-007-3040-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Table of contents (12 chapters)

  • Use of MAR Elements to Increase the Production of Recombinant Proteins

    Gorman, Cori (et al.)

    Pages 1-32

  • Expression Engineering – The IE2 Promoter/Enhancer from Mouse CMV

    Imhof, Markus O. (et al.)

    Pages 33-52

  • Defeating Randomness – Targeted Integration as a Boost for Biotechnology

    Gama-Norton, L. (et al.)

    Pages 53-82

  • Importance of Genetic Environment for Recombinant Gene Expression

    Dickson, Alan J.

    Pages 83-96

  • Expression Vector Engineering for Recombinant Protein Production

    Kim, Helen (et al.)

    Pages 97-108

Buy this book

eBook $199.00
price for USA (gross)
  • ISBN 978-90-481-2245-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $259.00
price for USA
  • ISBN 978-90-481-2244-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $259.00
price for USA
  • ISBN 978-94-007-3040-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Cell Line Development
Editors
  • Mohamed Al-Rubeai
Series Title
Cell Engineering
Series Volume
6
Copyright
2009
Publisher
Springer Netherlands
Copyright Holder
Springer Science+Business Media B.V.
eBook ISBN
978-90-481-2245-5
DOI
10.1007/978-90-481-2245-5
Hardcover ISBN
978-90-481-2244-8
Softcover ISBN
978-94-007-3040-3
Series ISSN
1389-6946
Edition Number
1
Number of Pages
VIII, 253
Topics