Targeted Genome Editing Using Site-Specific Nucleases

ZFNs, TALENs, and the CRISPR/Cas9 System

Editors: Yamamoto, Takashi (Ed.)

  • Equips readers with a basic understanding of genome editing methods, allowing for further developments in the field
  • Provides detailed methods for generation of engineered nucleases
  • Describes applications of genome editing methods in various organisms and in stem cells
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eBook $149.00
price for USA (gross)
  • ISBN 978-4-431-55227-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-4-431-55226-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 17, 2016
  • ISBN 978-4-431-56235-1
  • Free shipping for individuals worldwide
About this book

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Table of contents (13 chapters)

  • Genome Editing Using Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs)

    Ochiai, Hiroshi (et al.)

    Pages 3-24

  • CRISPR/Cas9: The Leading Edge of Genome Editing Technology

    Sakuma, Tetsushi (et al.)

    Pages 25-41

  • Editing Cultured Human Cells: From Cell Lines to iPS Cells

    Li, Hongmei Lisa (et al.)

    Pages 45-69

  • Genome Editing in Nematode

    Sugi, Takuma

    Pages 71-80

  • Highly Efficient Targeted Gene Disruption in the Silkworm, Bombyx mori, Using Genome Editing Tools

    Daimon, Takaaki

    Pages 81-96

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-4-431-55227-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-4-431-55226-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 17, 2016
  • ISBN 978-4-431-56235-1
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Targeted Genome Editing Using Site-Specific Nucleases
Book Subtitle
ZFNs, TALENs, and the CRISPR/Cas9 System
Editors
  • Takashi Yamamoto
Copyright
2015
Publisher
Springer Japan
Copyright Holder
Springer Japan
eBook ISBN
978-4-431-55227-7
DOI
10.1007/978-4-431-55227-7
Hardcover ISBN
978-4-431-55226-0
Softcover ISBN
978-4-431-56235-1
Edition Number
1
Number of Pages
VIII, 205
Number of Illustrations and Tables
12 b/w illustrations, 33 illustrations in colour
Topics