Springer Protocols Handbooks

Patch Clamp Techniques

From Beginning to Advanced Protocols

Editors: Okada, Yasunobu (Ed.)

  • Detailed descriptions of standard, essential, and widely used patch clamp (PC) techniques.
  • Practical descriptions of PC techniques for beginners and recently developed variations for experts.
  • The most up-to-date applications of PC, useful in academia and industry.
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eBook $109.00
price for USA (gross)
  • ISBN 978-4-431-53993-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-4-431-53992-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this Textbook

Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Reviews

From the reviews:

“This book is the most current single ‘how to’ manual and text on Patch clamp techniques. The whole cell, inside out patches, and membrane techniques are well illustrated. … The patch techniques for brain and spinal slices in culture has several chapters in this book. I recommend this to all those with an interest in biochemistry, membrane biology, neurophysiology, neurologists and neurosurgeons.” (Joseph J. Grenier, Amazon.com, May, 2014)


Table of contents (28 chapters)

  • Prologue: The Ion Channel

    Kukita, Fumio (et al.)

    Pages 1-19

  • Patch-Clamp Techniques: General Remarks

    Akita, Tenpei (et al.)

    Pages 21-41

  • Whole-Cell Patch Method

    Yawo, Hiromu

    Pages 43-69

  • Perforated Whole-Cell Patch-Clamp Technique: A User’s Guide

    Ishibashi, Hitoshi (et al.)

    Pages 71-83

  • Methods for Processing and Analyzing Single-Channel Data

    Sokabe, Masahiro

    Pages 85-104

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-4-431-53993-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-4-431-53992-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Patch Clamp Techniques
Book Subtitle
From Beginning to Advanced Protocols
Editors
  • Yasunobu Okada
Series Title
Springer Protocols Handbooks
Copyright
2012
Publisher
Springer Japan
Copyright Holder
Springer
eBook ISBN
978-4-431-53993-3
DOI
10.1007/978-4-431-53993-3
Hardcover ISBN
978-4-431-53992-6
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XI, 439
Topics