Experientia Supplementum

Proteomics in Functional Genomics

Protein Structure Analysis

Editors: Jolles, P., Jörnvall, Hans (Eds.)

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About this book

A wealth of information has accumulated over the last few years on the human genome. The new insights have completely changed the focus of protein analysis. It is no longer time-consuming analysis of unknown products, but rather selective identifications of individual forms, modifications and processings, and overall analysis of global protein outputs from cells and tissues in health and disease. This book gears to the rising need of sensitive, accurate, and fast separation and identification techniques in proteomics. It discusses current methodologies of modern protein analysis, from isolation and sample preparation, over analysis and identification, to final characterization. Several evaluations concentrate on the now productive approaches of two-dimensional gel electrophoresis and mass spectrometry, but alternative methods and further perspectives are also outlined. The book includes an overlook over current databases to connect protein analysis data with all available information,...

Table of contents (14 chapters)

  • Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis

    Appella, Ettore (et al.)

    Pages 1-27

  • Techniques for sample preparation including methods for concentrating peptide samples

    Gevaert, Kris (et al.)

    Pages 29-42

  • Sample preparation by SDS/PAGE and in-gel digestion

    Hellman, Ulf

    Pages 43-54

  • High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection

    Celis, Julio E. (et al.)

    Pages 55-67

  • Nanospray mass spectrometry in protein and peptide chemistry

    Griffiths, William J.

    Pages 69-79

Buy this book

eBook $309.00
price for USA (gross)
  • ISBN 978-3-0348-8458-7
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $389.00
price for USA
  • ISBN 978-3-7643-5885-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $389.00
price for USA
  • ISBN 978-3-0348-9576-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Proteomics in Functional Genomics
Book Subtitle
Protein Structure Analysis
Editors
  • P. Jolles
  • Hans Jörnvall
Series Title
Experientia Supplementum
Series Volume
88
Copyright
2000
Publisher
Birkhäuser Basel
Copyright Holder
Springer Basel AG
eBook ISBN
978-3-0348-8458-7
DOI
10.1007/978-3-0348-8458-7
Hardcover ISBN
978-3-7643-5885-3
Softcover ISBN
978-3-0348-9576-7
Series ISSN
1023-294X
Edition Number
1
Number of Pages
XIII, 236
Topics