Methods and Tools in Biosciences and Medicine

Methods in Non-Aqueous Enzymology

Editors: Gupta, Munishwar N. (Ed.)

Buy this book

eBook $239.00
price for USA (gross)
  • ISBN 978-3-0348-8472-3
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $399.00
price for USA
  • ISBN 978-3-7643-5803-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $309.00
price for USA
  • ISBN 978-3-7643-6109-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac­ tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol­ ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol­ ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac­ tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.

Table of contents (12 chapters)

  • Non-Aqueous Enzymology: Issues and Persspectives

    Munishwar N. Gupta

    Pages 1-13

  • Importance of Water Activity for Enzyme Catalysis in Non-Aqueous Organic Systems

    Anthonsen, Thorleif (et al.)

    Pages 14-35

  • Engineering of Enzymes via Immobili-zation and Post-Immobilization Techniques: Preparation of Enzyme Derivatives with Improved Stability in Organic Media

    Fernández-Lorente, Gloria (et al.)

    Pages 36-51

  • Immobilization of Lipases for Use in Non-Aqueous Reaction Systems

    Bosley, John A. (et al.)

    Pages 52-69

  • Applications of Enzymes and Membrane Technology in Fat and Oil Processing Processing

    Nakajima, Mitsutoshi (et al.)

    Pages 70-89

Buy this book

eBook $239.00
price for USA (gross)
  • ISBN 978-3-0348-8472-3
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $399.00
price for USA
  • ISBN 978-3-7643-5803-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $309.00
price for USA
  • ISBN 978-3-7643-6109-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Methods in Non-Aqueous Enzymology
Editors
  • Munishwar N. Gupta
Series Title
Methods and Tools in Biosciences and Medicine
Copyright
2000
Publisher
Birkhäuser Basel
Copyright Holder
Springer Basel AG
eBook ISBN
978-3-0348-8472-3
DOI
10.1007/978-3-0348-8472-3
Hardcover ISBN
978-3-7643-5803-7
Softcover ISBN
978-3-7643-6109-9
Edition Number
1
Number of Pages
X, 218
Topics