Skip to main content
SpringerLink
Log in

Hydrocarbon and Lipid Microbiology Protocols

Isolation and Cultivation

  • Book
  • © 2017

Overview

Editors:
  1. Terry J. McGenity

    1. School of Biological Sciences, University of Essex, Colchester, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Kenneth N. Timmis

    1. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Balbina Nogales

    1. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Offers readily reproducible, step-by-step laboratory methods
  • Provides helpful tips and tricks to complement the protocols
  • Includes troubleshooting advice
  • Features practical laboratory guidelines to promote successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to the Isolation and Cultivation of Microbes Involved in the Hydrocarbon Cycle

    • Terry J. McGenity
    Pages 1-25

  3. Protocols for High-Throughput Isolation and Cultivation

    • Karsten Zengler
    Pages 27-35

  4. Single Bacteria Studies Using Microfluidics

    • Yanqing Song, Bing Li, Yong Qiu, Huabing Yin
    Pages 37-55

  5. Cultivating Fastidious Microbes

    • J. Cameron Thrash, Jessica Lee Weckhorst, David M. Pitre
    Pages 57-78

  6. Cultivation and Preservation of Hydrocarbonoclastic Microorganisms, Particularly Cycloclasticus Species

    • Maria Genovese, Renata Denaro, Daniela Russo, Francesca Crisafi, Santina Santisi, Simone Cappello et al.
    Pages 79-93

  7. Cultivating Aerobic Hydrocarbon-Degrading Bacteria from Micro-algae

    • Tony Gutierrez
    Pages 95-106

  8. Two-Phase Cultivation Techniques for Hydrocarbon-Degrading Microorganisms

    • Lukas Y. Wick, Sally Otto, Christof Holliger
    Pages 107-117

  9. Protocol for Isolation, Screening, and Cultivation of Asphaltene-Degrading Microorganism

    • Seyed Morteza Zamir, Tina Tavassoli, Seyed Abbas Shojaosadati
    Pages 119-129

  10. Cultivation of Anaerobic Microorganisms with Hydrocarbons as Growth Substrates

    • F. Widdel
    Pages 131-142

  11. Enrichment and Isolation of Metal Respiring Hydrocarbon Oxidizers

    • Adam J. Williamson, John D. Coates
    Pages 143-164

  12. Enrichment and Isolation of Chloroxyanion-Respiring Hydrocarbon Oxidizers

    • Tyler P. Barnum, John D. Coates
    Pages 165-176

  13. Cultivation of Methanogens

    • Taiki Katayama, Yoichi Kamagata
    Pages 177-195

  14. Cultivation of Methylotrophs

    • Donovan P. Kelly, Julie K. Ardley, Ann P. Wood
    Pages 197-229

  15. Cultivation of Methanotrophs

    • Svetlana N. Dedysh, Peter F. Dunfield
    Pages 231-247

  16. Cultivation of Environmental Bacterial Communities as Multispecies Biofilms

    • Marc A. Demeter, Joe Lemire, Susanne Golby, Monika Schwering, Howard Ceri, Raymond J. Turner
    Pages 249-268

  17. Cultivation of Hydrocarbon-Degrading Fungi

    • Dietmar Schlosser, Lukas Y. Wick
    Pages 269-281

  18. Oleaginous Microalgae Isolation and Screening for Lipid Productivity Using a Standard Protocol

    • Van Thang Duong, Boer Bao, Peer M. Schenk
    Pages 283-298

  19. Preservation of Microbial Pure Cultures and Mixed Communities

    • Bram Vekeman, Kim Heylen
    Pages 299-315

  20. Erratum to: Protocols for High-Throughput Isolation and Cultivation

    • Karsten Zengler
    Pages 317-317
Back to top

Keywords

About this book

This Volume describes methods for cultivating hydrocarbon-producing and -consuming microbes, covering compounds in a range of states – gaseous (e.g. methane), liquid (e.g. alkanes of intermediate molecular weight) and solid (e.g. many PAHs and asphaltene). It also examines the cultivation of aerobic and anaerobic hydrocarbon degraders using a range of electron acceptors (e.g. oxygen, nitrate, sulphate, metals, (per)chlorate), and a separate chapter is devoted to explaining the cultivation of methanogens. Special attention is given to: high-throughput cultivation, growing microbes as biofilms, and cultivating fastidious microbes, as well as the preservation of microbial pure cultures and consortia. Accordingly, this Volume will be of value to anyone embarking on the selective enrichment and cultivation of novel microorganisms.


Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.







Editors and Affiliations

  • School of Biological Sciences, University of Essex, Colchester, United Kingdom

    Terry J. McGenity

  • Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  • Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

Bibliographic Information

  • Book Title: Hydrocarbon and Lipid Microbiology Protocols

  • Book Subtitle: Isolation and Cultivation

  • Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-662-45179-3

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag GmbH Germany, part of Springer Nature 2017

  • Hardcover ISBN: 978-3-662-45178-6Published: 15 June 2017

  • Softcover ISBN: 978-3-662-56878-1Published: 12 May 2018

  • eBook ISBN: 978-3-662-45179-3Published: 07 June 2017

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 319

  • Number of Illustrations: 30 b/w illustrations, 20 illustrations in colour

  • Topics: Microbiology, Biochemical Engineering

Publish with us

Policies and ethics

Back to top

Search

Navigation