Results and Problems in Cell Differentiation

Differentiation of Protoplasts and of Transformed Plant Cells

Editors: Reinert, J., Binding, H. (Eds.)

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About this book

H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma­ nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protoplasts represent cells which are liberated from their walls and separated from the differentiation pattern of the organism. Investigations on re­ generation from protoplasts provide a better understanding of the process and control of developmental pathways. Whereas protoplast isolation results in alteration of the state of differentiation of a cell, protoplast fusion is a means for the creation of cells with novel genetic constitution. Fascinating features are (1) to hybridize cells which-unlike gametes -did not derive from meiosis products, (2) to bring together foreign plastids and mitochondria and to investigate their parasexual reactions, and (3) to match ge­ netic traits which had been separated for long periods of evolution. Highly sophisticated techniques have already been elaborated for the transfer of genes by the use of isolated DNA and gene transfer systems. Highly promising results have already been obtained by the use of Ti plasmids of Agrobacterium, but direct DNA transformation is also proving to be useful. Most of the results in these areas are preliminary and/or limited to a few sys­ tem.lt is the aim of this volume to present the main features, but at the same time to draw attention to problems and perspectives of protoplast regeneration and so­ matic cell genetics in order to stimulate further investigations.

Table of contents (6 chapters)

  • Introduction

    Binding, H. (et al.)

    Pages 1-1

  • Isolation and Regeneration of Protoplasts from Higher Plants

    Maheshwari, S. C. (et al.)

    Pages 3-36

  • Protoplast Fusion and Early Development of Fusants

    Binding, H. (et al.)

    Pages 37-66

  • Development of Protoplast Fusion Products

    Nehls, R. (et al.)

    Pages 67-108

  • Molecular Biology of Plant Cell Transformation

    Yadav, N. S.

    Pages 109-142

Buy this book

eBook $74.99
price for USA (gross)
  • ISBN 978-3-540-39836-3
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.00
price for USA
  • ISBN 978-3-662-21816-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Differentiation of Protoplasts and of Transformed Plant Cells
Editors
  • J. Reinert
  • H. Binding
Series Title
Results and Problems in Cell Differentiation
Series Volume
12
Copyright
1986
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-540-39836-3
DOI
10.1007/978-3-540-39836-3
Softcover ISBN
978-3-662-21816-7
Series ISSN
0080-1844
Edition Number
1
Number of Pages
VIII, 157
Number of Illustrations and Tables
11 b/w illustrations
Topics