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Micromethods in Molecular Biology

  • Book
  • © 1973

Overview

Authors:
  1. Volker Neuhoff

    1. Medizin (Arbeitsgruppe Neurochemie), Max-Planck-Institut für Experimentelle, Göttingen, Germany

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-XV
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  2. Micro-Electrophoresis on Polyacrylamide Gels

    • Volker Neuhoff
    Pages 1-83

  3. Micro-Determination of Amino Acids and Related Compounds with Dansyl Chloride

    • Volker Neuhoff
    Pages 85-147

  4. Micro-Determination of Phospholipids

    • Volker Neuhoff
    Pages 149-178

  5. Micro-Diffusion Techniques

    • Volker Neuhoff
    Pages 179-203

  6. Capillary Centrifugation

    • Volker Neuhoff
    Pages 205-213

  7. Micro-Electrophoresis for RNA and DNA Base Analysis

    • Volker Neuhoff
    Pages 215-256

  8. Determination of the Dry Mass of Small Biological Objects by Quantitative Electron Microscopy

    • Volker Neuhoff
    Pages 257-284

  9. The Construction and Use of Quartz Fiber Fish Pole Balances

    • Volker Neuhoff
    Pages 285-295

  10. Microphotometry

    • Volker Neuhoff
    Pages 297-328

  11. Cytofluorometry

    • Volker Neuhoff
    Pages 329-346

  12. Quantitative Autoradiography at the Cellular Level

    • Volker Neuhoff
    Pages 347-393

  13. Micro-Dialysis

    • Volker Neuhoff
    Pages 395-398

  14. Micro-Homogenisation

    • Volker Neuhoff
    Pages 399-402

  15. Wet Weight Determination in the Lower Milligram Range

    • Volker Neuhoff
    Pages 403-404

  16. Micro-Magnetic Stirrer

    • Volker Neuhoff
    Pages 405-406

  17. Production of Capillary Pipettes

    • Volker Neuhoff
    Pages 407-409

  18. Back Matter

    Pages 411-430
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About this book

This book is based on practical experience and is therefore written as a prac­ tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli­ cation of the micromethods. There are many other sensitive and excellent micro­ methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi­ ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.

Authors and Affiliations

  • Medizin (Arbeitsgruppe Neurochemie), Max-Planck-Institut für Experimentelle, Göttingen, Germany

    Volker Neuhoff

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