Trafficking of Intracellular Membranes:

1. Front Matter

   Pages I-IX

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2. Protein-Lipid and Lipid-Lipid interactions in model systems and in biological membranes

   • A. Bienvenüe, J. Sainte-Marie, L. Maurin

   Pages 1-10

3. Dynamical Properties of Membranes: Application of Fluorescent Lipid Analogs

   • Dick Hoekstra, Teresa Babia, Mirjam Zegers, Kristien Zaal, Eugene G. J. M. Arts, Jan Willem Kok

   Pages 11-33

4. The Erythrocyte Aminophospholipid Translocase

   • Alan J. Schroit

   Pages 35-48

5. Purification and Substrate Specificity of the Human Erythrocyte Aminophospholipid Transporter

   • David L. Daleke, Jill V. Lyles, Edward Nemergut, Michael L. Zimmerman

   Pages 49-59

6. Phospholipids in Platelets: Localization, Movement and Physiological Function

   • Alain Bienvenüe, Patrick Gaffet, Nadir Bettache

   Pages 61-69

7. Interactions of Peptides with Phospholipid Vesicles: Fusion, Leakage and Flip-Flop

   • Shlomo Nir, Elias Fattal, Roberta A. Parente, Jose L. Nieva, Jan Wilschut, Francis C. Szoka Jr.

   Pages 71-96

8. Molecular Mechanisms of Membrane Fusion

   • Nejat Düzgüneş§

   Pages 97-129

9. Entry of Enveloped Viruses Into Host Cells: Fusion Activity of the Influenza Virus Hemagglutinin

   • Maria C. Pedroso de Lima, João Ramalho-Santos, Nejat Düzgünes, Diana Flasher, Shlomo Nir

   Pages 131-154

10. Mass action model of virus fusion

    • Shlomo Nir, Nejat Düzgüneş, Dick Hoekstra, João Ramalho-Santos, Maria C. Pedroso de Lima

    Pages 155-170

11. Viral Membrane Proteins as Tools to Study Protein Folding, Assembly, and Transport

    • Robert W. Doms, Stephen T. Abedon, Thomas M. Richardson Jr.

    Pages 171-186

12. Membrane Fusion Induced by the HIV env Glycoprotein: Purification of CD4 for Reconstitution Studies

    • Charles Larsen, Arun Patel, Joe Bentz

    Pages 187-199

13. Cycling of Rab Proteins: Role of Rab GDI in the Reversible Membrane Association of Rab GTP-Ases

    • Oliver Ullrich, Marino Zerial

    Pages 201-209

14. Microtubule Dependent Transport and Fusion of Phagosomes with the Endocytic Pathway

    • Janis K. Burkhardt, Ariel Blocker, Andrea Jahraus, Gareth Griffiths

    Pages 211-222

15. GTPases: Key regulatory components of the endocytic pathway

    • M. Alejandro Barbieri, Maria Isabel Colombo, Guangpu Li, Luis Segundo Mayorga, Philip Stahl

    Pages 223-234

16. Glycolipids of Cellular Surfaces: Topology of Metabolism, Function and Pathobiochemistry of Glycolipid Binding Proteins

    • Konrad Sandhoff, Gerhild van Echten-Deckert

    Pages 235-246

17. Niemann-Pick Disease Type A and B- Natural History Of Lysosomal Sphingomyelinase

    • Klaus Feriinz, Konrad Sandhoff

    Pages 247-263

18. Interaction of liposomes with cells in vitro

    • Kyung-Dall Lee, Demetrios Papahadjopoulos

    Pages 265-284

19. Molecular Analysis of Exocytosis in Neurons and Endocrine Cells

    • Barbara Höhne-Zell, Manfred Gratzl

    Pages 285-301

20. Control of Intracellular Free Calcium in Neurons and Endocrine Cells

    • Karl Josef Föhr, Artur Mayerhofer, Manfred Gratzl

    Pages 303-315

This volume contains the lectures presented at the NATO Advanced Study Institute (ASI) on "Trafficking of Intracellular Membranes: From Molecular Sorting to Membrane Fusion", held in Espinho, Portugal, from June 19 to June 30,1994. The objective of this Institute was to survey recent developments and to discuss future directions in the rapidly advancing field of membrane cell biology, with particular emphasis on the dynamical properties and intracellular flow of membranes. A wide range of interrelated topics around the central theme of intracellular trafficking of membranes was covered, including lipid flow, membrane fusion, dynamics of membrane components, protein folding and assembly, vesicular transport in membrane biogenesis, exocytosis and endocytosis. A large variety of experimental techniques and systems, including the application of viruses and model systems, to study these processes was also considered. Membrane cell biology is a broad discipline which encompasses many scientific areas including cell biology, biochemistry, biophysics, virology, immunonology and genetics. Indeed, recent advances in the cell biology of membranes could not have been made without this multidisciplinary approach. Significant progress achieved during the last few years in understanding how newly synthesized lipids and proteins find their way to the cell organelles, how molecular sorting and the continuous flow of membranes allow each cellular membrane to maintain its own distinct molecular composition, and, thereby, the individuality of the various intracellular compartments, was discussed in considerable detail in this Institute.

• Calcium
• Lipid
• biochemistry
• biomembrane
• cell
• glycoprotein
• membrane
• membrane transport
• metabolism
• oncogene
• pathobiochemistry
• protein
• protein folding
• proteins
• receptor

• Departamento de Bioquimica, Universidade de Coimbra, Coimbra, Portugal

  Maria C. Pedroso Lima

• Department of Microbiology, University of the Pacific, San Francisco, USA

  Nejat Düzgüneş

• Department of Physiological Chemistry, University of Groningen, Groningen, The Netherlands

  Dick Hoekstra

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