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Measuring Calcium and Calmodulin Inside and Outside Cells

  • Book
  • © 2001

Overview

Editors:
  1. Ole H. Petersen

    1. Department of Physiology, The University of Liverpool, Liverpool, UK

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  • Step by step protocols of the various techniques for measuring Ca2+ inside and outside cells and organelles
  • Protocols contain the experience of researchers who did pioneering work in the field of measuring Ca2+ and tracking calmodulin inside cells

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (13 chapters)

  1. Front Matter

    Pages I-IX
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  2. Cytosolic Ca2+ Concentration Measurements

    1. Front Matter

      Pages 1-1
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    2. Principles of Fluorescence Measurements — Dyes and Hardware Required

      • Emil C. Toescu, Alexej Verkhratsky
      Pages 3-45

    3. Simple Principles of Imaging — Image Files, Image Processing and Image Analysis

      • Emil C. Toescu
      Pages 47-64

    4. Aequorin Measurements of Cytosolic Ca2+

      • C. Jane Dixon, Anne K. Green
      Pages 65-89

    5. Electrophysiological Measurements Using Ca2+-Sensitive Microelectrodes

      • Roger C. Thomas
      Pages 91-102

  3. Measuring Ca2+ Inside Organelles

    1. Front Matter

      Pages 103-103
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    2. Measuring Ca2+ in the Nucleoplasm of Intact Cells

      • Marisa Brini, Paolo Pinton, Paulo Magalhães, Luisa Filippin, Tullio Pozzan, Rosario Rizzuto
      Pages 105-130

    3. Measuring Ca2+ in Isolated Nuclei

      • Oleg Gerasimenko
      Pages 131-145

    4. Ca2+ Measurement with Luminescent Probes in the Endoplasmic Reticulum

      • Javier Alvarez, Mayte Montero
      Pages 147-163

    5. Ca2+ Measurement with Fluorescent Probes in the Endoplasmic Reticulum

      • Aldebaran M. Hofer
      Pages 165-185

    6. Ca2+ Measurements in Mitochondria

      • Paolo Pinton, Robert Drummond, Paulo Magalhães, Marisa Brini, Anna Chiesa, Tullio Pozzan et al.
      Pages 187-211

    7. Ca2+ Measurements in Secretory Granules

      • Oleg Gerasimenko
      Pages 213-229

    8. Ca2+ Measurement in Endosomes of Intact Cells

      • Julia V. Gerasimenko
      Pages 231-247

  4. Misellaneous

    1. Front Matter

      Pages 249-249
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    2. Measuring Ca2+ Extrusion from Single Cells

      • Pavel V. Belan
      Pages 251-266

    3. Tracking Calmodulin Inside Cells

      • Madeleine Craske
      Pages 267-290

  5. Back Matter

    Pages 291-301
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Keywords

About this book

The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signalling molecules as well as very many pathophysiological processes including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles and in the immediate surroundings of individual cells are described in practical details by experts, on the basis of their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.

Editors and Affiliations

  • Department of Physiology, The University of Liverpool, Liverpool, UK

    Ole H. Petersen

Bibliographic Information

  • Book Title: Measuring Calcium and Calmodulin Inside and Outside Cells

  • Editors: Ole H. Petersen

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-56851-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2001

  • Softcover ISBN: 978-3-642-47737-9Published: 15 April 2014

  • eBook ISBN: 978-3-642-56851-0Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: IX, 301

  • Number of Illustrations: 101 b/w illustrations, 6 illustrations in colour

  • Topics: Biochemistry, general, Human Physiology, Cell Biology

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