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Genetic Preservation of Plant Cells in Vitro

  • Book
  • © 1995

Overview

Editors:
  1. Brian Grout

    1. Crisp Innovar Ltd., Reepham, Norfolk, UK

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Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (8 chapters)

  1. Front Matter

    Pages I-XI
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  2. Introduction to the in Vitro Preservation of Plant Cells, Tissues and Organs

    • B. W. W. Grout
    Pages 1-20

  3. Minimal Growth Storage

    • B. W. W. Grout
    Pages 21-27

  4. Cryopreservation of Protoplast, Suspension and Callus Cultures

    • B. W. W. Grout
    Pages 29-46

  5. Cryopreservation of Excised Meristems, Shoot Tips and Somatic Embryos

    • B. W. W. Grout
    Pages 47-61

  6. Storage of Free Pollen, Pollen Embryos and the Zygotic Embryos of Seed by Cryopreservation and Freeze Drying

    • B. W. W. Grout, A. V. Roberts
    Pages 63-74

  7. Conservation of Algae

    • J. G. Day, M. R. McLellan
    Pages 75-98

  8. Cryopreservation by Vitrification

    • L. E. Towill
    Pages 99-111

  9. Biochemical and Molecular Methods for Assessing Damage, Recovery and Stability in Cryopreserved Plant Germplasm

    • K. Harding, E. E. Benson
    Pages 113-169

  10. Back Matter

    Pages 171-171
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Keywords

About this book

The long-term storage and maintenance of viable plant cells and organs is an area of active concern across the range of pure and applied plant sciences. In academic, government and commercial laboratories, the extended storage of propagules of one sort or another, with maximum protection of the genome from mutation and altered expression, is often a very necessary activity that can draw heavily on resources and effort. However, preservation per se is typically not an activity in its own right, but a facilitating technology that is part of a larger programme of work. Consequently, there are many laboratories that do not have the benefit of a specialist in storage technology, and have to delegate the responsibility to individuals, or teams, who are faced with a daunting learning curve. To maximise the chances of success, in the shortest possible time and with minimum losses, these researchers need sources of reference that are au­ thoritative and soundly based in practical experience.

Reviews

"Each chapter is written as a "hands-on" practical guide and presents procedures that can be adapted to individual laboratory conditions and modified to best suit the particular material being preserved." Bioscope

Editors and Affiliations

  • Crisp Innovar Ltd., Reepham, Norfolk, UK

    Brian Grout

Bibliographic Information

  • Book Title: Genetic Preservation of Plant Cells in Vitro

  • Editors: Brian Grout

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-78661-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-642-48968-6Published: 01 July 2012

  • eBook ISBN: 978-3-642-78661-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XI, 169

  • Number of Illustrations: 22 b/w illustrations

  • Topics: Biochemistry, general, Plant Sciences, Cell Biology, Agriculture, Forestry

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