SpringerBriefs in Applied Sciences and Technology

Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology

Authors: Malboubi, Majid, Jiang, Kyle

  • Explains the mechanisms of gigaseal formation to the latest level of discoveries
  • Provides practical techniques, which enable frequent formation of high resistance seals
  • Shares authors´ recent practice in acquiring gigaseal formation using micropipettes
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eBook $39.99
price for USA (gross)
  • ISBN 978-3-642-39128-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $49.99
price for USA
  • ISBN 978-3-642-39127-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Rent the ebook  
  • Rental duration: 1 or 6 month
  • low-cost access
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About this book

This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.

Reviews

“This is a very good book on patch clamp protocol with a focus on the engineering and physics of pipettes. A description on tuning for bore diameter and various approaches to the culture dishes is detailed. … This is a reliable resource for use in the physiology labs. Physiologists, students, postdocs, & senior investigators will find this very helpful.” (Joseph J. Grenier, Amazon.com, September, 2015)


Table of contents (7 chapters)

  • Introduction

    Malboubi, Majid (et al.)

    Pages 1-2

  • Development of Patch Clamping

    Malboubi, Majid (et al.)

    Pages 3-16

  • Gigaseal Formation

    Malboubi, Majid (et al.)

    Pages 17-28

  • Effect of Roughness on Gigaseal Formation

    Malboubi, Majid (et al.)

    Pages 29-47

  • Effect of Hydrophilicity on Gigaseal Formation

    Malboubi, Majid (et al.)

    Pages 49-59

Buy this book

eBook $39.99
price for USA (gross)
  • ISBN 978-3-642-39128-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $49.99
price for USA
  • ISBN 978-3-642-39127-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Rent the ebook  
  • Rental duration: 1 or 6 month
  • low-cost access
  • online reader with highlighting and note-making option
  • can be used across all devices
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Bibliographic Information

Bibliographic Information
Book Title
Gigaseal Formation in Patch Clamping
Book Subtitle
With Applications of Nanotechnology
Authors
Series Title
SpringerBriefs in Applied Sciences and Technology
Copyright
2014
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
The Author(s)
eBook ISBN
978-3-642-39128-6
DOI
10.1007/978-3-642-39128-6
Softcover ISBN
978-3-642-39127-9
Series ISSN
2191-530X
Edition Number
1
Number of Pages
XI, 94
Number of Illustrations and Tables
16 b/w illustrations, 65 illustrations in colour
Topics