Springer Protocols Handbooks

Advanced Protocols for Animal Transgenesis

An ISTT Manual

Editors: Pease, Shirley, Saunders, Thomas L. (Eds.)

  • Provides step-by-step instructions and helpful troubleshooting notes
  • Published in cooperation with the International Society for Transgenic Technology (ISTT)
  • With contributions by leading scientists
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Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-3-642-20792-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
valid through November 5, 2017
  • ISBN 978-3-642-20791-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Table of contents (26 chapters)

  • Patent and Licensing Issues in Transgenic Technology

    Canady, Karen S.

    Pages 1-23

  • Global Resources: Including Gene Trapped ES Cell Clones - Is Your Gene Already Knocked Out?

    Montoliu, Lluis

    Pages 25-42

  • Designing Transgenes for Optimal Expression

    Moltó, Eduardo (et al.)

    Pages 43-56

  • Gene Targeting Vector Design for Embryonic Stem Cell Modifications

    Saunders, Thomas L.

    Pages 57-79

  • Transgenic Production Benchmarks

    Fielder, Thomas J. (et al.)

    Pages 81-97

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-3-642-20792-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
valid through November 5, 2017
  • ISBN 978-3-642-20791-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Advanced Protocols for Animal Transgenesis
Book Subtitle
An ISTT Manual
Editors
  • Shirley Pease
  • Thomas L. Saunders
Series Title
Springer Protocols Handbooks
Copyright
2011
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-20792-1
DOI
10.1007/978-3-642-20792-1
Hardcover ISBN
978-3-642-20791-4
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XV, 669
Number of Illustrations and Tables
145 b/w illustrations, 65 illustrations in colour
Topics