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Restriction Endonucleases

  • Book
  • © 2004

Overview

Editors:
  1. Alfred M. Pingoud

    1. Institute for Biochemistry, Justus-Liebig-University, Giessen, Germany

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  • First book dealing with all aspects of restriction endonucleases
  • Includes supplementary material: sn.pub/extras

Part of the book series: Nucleic Acids and Molecular Biology (NUCLEIC, volume 14)

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Table of contents (16 chapters)

  1. Front Matter

    Pages I-XXVI
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  2. A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes

    • R. J. Roberts, M. Belfort, T. Bestor, A. S. Bhagwat, T. A. Bickle, J. Bitinaite et al.
    Pages 1-18

  3. Restriction-Modification Systems as Minimal Forms of Life

    • I. Kobayashi
    Pages 19-62

  4. Molecular Phylogenetics of Restriction Endonucleases

    • J. M. Bujnicki
    Pages 63-93

  5. Sliding or Hopping? How Restriction Enzymes Find Their Way on DNA

    • A. Jeltsch, C. Urbanke
    Pages 95-110

  6. The Type I and III Restriction Endonucleases: Structural Elements in Molecular Motors that Process DNA

    • S. E. McClelland, M. D. Szczelkun
    Pages 111-135

  7. The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease

    • A. Grigorescu, M. Horvath, P. A. Wilkosz, K. Chandrasekhar, J. M. Rosenberg
    Pages 137-177

  8. Structure and Function of EcoRV Endonuclease

    • F. K. Winkler, A. E. Prota
    Pages 179-214

  9. Two of A Kind: BamHI and BglII

    • É. Scheuring Vanamee, H. Viadiu, C. M. Lukacs, A. K. Aggarwal
    Pages 215-236

  10. Structure and Function of the Tetrameric Restriction Enzymes

    • V. Siksnys, S. Grazulis, R. Huber
    Pages 237-259

  11. Structure and Function of Type IIE Restriction Endonucleases — or: From a Plasmid That Restricts Phage Replication to A New Molecular DNA Recognition Mechanism

    • M. Reuter, M. Mücke, D. H. Krüger
    Pages 261-295

  12. Analysis of Type II Restriction Endonucleases that Interact with Two Recognition Sites

    • A. J. Welsh, S. E. Halford, D. J. Scott
    Pages 297-317

  13. The Role of Water in the EcoRI-DNA Binding

    • N. Sidorova, D. C. Rau
    Pages 319-337

  14. Role of Metal Ions in Promoting DNA Binding and Cleavage by Restriction Endonucleases

    • J. A. Cowan
    Pages 339-360

  15. Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions in DNA Cleavage

    • J. R. Horton, R. M. Blumenthal, X. Cheng
    Pages 361-392

  16. Protein Engineering of Restriction Enzymes

    • J. Alves, P. Vennekohl
    Pages 393-411

  17. Engineering and Applications of Chimeric Nucleases

    • K. Kandavelou, M. Mani, S. Durai, S. Chandrasegaran
    Pages 413-434

  18. Back Matter

    Pages 435-443
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Keywords

About this book

Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

Editors and Affiliations

  • Institute for Biochemistry, Justus-Liebig-University, Giessen, Germany

    Alfred M. Pingoud

Bibliographic Information

  • Book Title: Restriction Endonucleases

  • Editors: Alfred M. Pingoud

  • Series Title: Nucleic Acids and Molecular Biology

  • DOI: https://doi.org/10.1007/978-3-642-18851-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2004

  • Hardcover ISBN: 978-3-540-20502-9Published: 19 February 2004

  • Softcover ISBN: 978-3-642-62324-0Published: 21 September 2012

  • eBook ISBN: 978-3-642-18851-0Published: 06 December 2012

  • Series ISSN: 0933-1891

  • Series E-ISSN: 1869-2486

  • Edition Number: 1

  • Number of Pages: XXVI, 443

  • Topics: Biochemistry, general, Biotechnology, Cell Biology, Microbiology

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