Springer Lab Manuals

Peptide Arrays on Membrane Supports

Synthesis and Applications

Editors: Koch, Joachim, Mahler, Michael (Eds.)

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  • ISBN 978-3-662-09229-3
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Softcover $289.00
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  • ISBN 978-3-540-42532-8
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Softcover $289.00
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  • ISBN 978-3-642-07639-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.

Reviews

From the reviews:

"In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. … It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding." (New Drugs, Issue 7, October, 2002)

"Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications … . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students." (Biotech International, Vol. 15 (2), 2003)


Table of contents (11 chapters)

  • SPOT Synthesis — Scope of Applications

    Frank, Ronald (et al.)

    Pages 1-22

  • Chemistry of Fmoc Peptide Synthesis on Membranes

    Zander, Norbert (et al.)

    Pages 23-39

  • Manual Peptide Synthesis

    Petersen, Gabriele

    Pages 41-53

  • Automated Synthesis of Solid-Phase Bound Peptides

    Gausepohl, Heinrich (et al.)

    Pages 55-68

  • Epitope Mapping of Antibodies with Solid-Phase Oligopeptides

    Koch, Joachim (et al.)

    Pages 69-82

Buy this book

eBook $229.00
price for USA (gross)
  • ISBN 978-3-662-09229-3
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $289.00
price for USA
  • ISBN 978-3-540-42532-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $289.00
price for USA
  • ISBN 978-3-642-07639-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Peptide Arrays on Membrane Supports
Book Subtitle
Synthesis and Applications
Editors
  • Joachim Koch
  • Michael Mahler
Series Title
Springer Lab Manuals
Copyright
2002
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-09229-3
DOI
10.1007/978-3-662-09229-3
Softcover ISBN
978-3-540-42532-8
Softcover ISBN
978-3-642-07639-8
Edition Number
1
Number of Pages
X, 173
Number of Illustrations and Tables
23 b/w illustrations
Topics