Biological and Medical Physics, Biomedical Engineering

Membrane Proteins in Aqueous Solutions

From Detergents to Amphipols

Authors: Popot, Jean-Luc

  • Written by Jean-Luc Popot, one of the foremost experts in the field
  • First book dedicated to an emerging methodology, namely the utilization for amphipols and their interaction with membrane proteins
  • Essential attributes are summarized in many richly-illustrated figures and tables
  • Includes a Foreword by Donald M. Engelman and over 30 cartoons illustrated by Francis Haraux
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Buy this book

eBook $169.00
price for USA (gross)
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: April 14, 2018
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format:
  • ebooks can be used on all reading devices
Hardcover $219.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: March 17, 2018
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
About this book

Membrane proteins represent about one third of the proteins encoded in a cell's genome, and, because of their key physiological roles, more than half of drug targets. Detergents are traditionally used to extract proteins from membranes in order to make them amenable to biochemical and biophysical approaches. However, detergent-solubilized proteins are generally unstable. This has led to the development of alternative, non-conventional surfactants, such as bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants, and specially designed amphipathic polymers called 'amphipols'. These novel tools, mainly developed over the past 20 years, are revolutionizing handling membrane proteins in vitro for basic and applied research, as well as for such biomedical applications as drug screening or vaccination.
This book, written by a specialist of membrane proteins and one of the creators of amphipols, describes the properties and uses of these novel molecules. It opens with general introductions on membrane proteins and their natural environment, detergents, the current status of membrane protein in vitro studies, a broad panorama of non-conventional surfactants and a discussion of their respective advantages and limitations, and the preparation and properties of amphipols and membrane protein/amphipol complexes. Topical chapters cover in vitro folding, cell-free synthesis and stabilization of membrane proteins, and such biophysical and biochemical applications as electron microscopy, X-ray diffraction, NMR, optical spectroscopy, mass spectrometry, the whole range of solutions studies, proteomics, and such practical applications as membrane protein immobilization and drug screening and the use of amphipols in vivo for vaccination and drug delivery. Each topical chapter is introduced with a concise, up-to-date overview of how membrane proteins are currently studied using each individual technique, before offering an exhaustive coverage and in-depth discussion of the contribution of amphipols, and concluding with hands-on protocols written by everyday practitioners of each application.
In addition to a comprehensive coverage of the properties and uses of non-conventional surfactants, this book therefore also offers a concise, accessible introduction to membrane protein biochemistry and biophysics. It is meant to be used both in basic and applied research laboratories and as a teaching help.

About the authors

Jean-Luc Popot, born 1948, studied biology, chemistry, and biophysics in Orléans and Orsay. In 1971, he took a permanent position with the Collège de France. In J.‑P. Changeux’s laboratory, at the Pasteur Institute, he applied electrophysiology and biophysical and biochemical approaches to functional and structural studies of the nicotinic acetyl­cho­li­ne receptor. In 1982, he joined D.M. Engelman at Yale University as a visiting scientist. His work at Yale, which bore princi­pal­ly on refolding bacterio­rhod­opsin from dena­tu­red fragments and studying the refolded structure crystallographically, led the two of them to propose, in 1990, an influential model for the folding of α‑helical membrane proteins. In 1985, he joined the laboratory of P. Joliot at the Institut de Biologie Physico-Chimique (Paris). His group car­ried out neutron diffraction and model building work on bacterio­rhod­opsin and bio­che­mical studies on Photo­sys­tem II and cytochrome bf. In 1996, he became Research Director at the Centre National de la Recher­­che Scienti­fi­que and created his own laboratory, where the X‑ray struc­tu­re of the bf was solved in 2003. In parallel, he pursued the development of sequence analysis approa­ches and, in collabo­ra­tion with chemists and physical chemists, designed and validated non-conventional surfact­ants aimed at facilitating membrane protein solu­tion studies, most notably amphi­­pathic polymers (‘amphipols’) and fluorinated surfactants. He retired in 2013 and, along with his wife, splits his time between restoring an old house in Languedoc, reading, writing, hiking, photographing, cooking, and enjoying the company of kith and kin.

Buy this book

eBook $169.00
price for USA (gross)
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: April 14, 2018
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format:
  • ebooks can be used on all reading devices
Hardcover $219.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: March 17, 2018
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Proteins in Aqueous Solutions
Book Subtitle
From Detergents to Amphipols
Authors
Series Title
Biological and Medical Physics, Biomedical Engineering
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing AG
eBook ISBN
978-3-319-73148-3
DOI
10.1007/978-3-319-73148-3
Hardcover ISBN
978-3-319-73146-9
Series ISSN
1618-7210
Edition Number
1
Number of Pages
VIII, 842
Number of Illustrations and Tables
132 b/w illustrations, 220 illustrations in colour
Topics