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Springer Theses

Rapid Cell Magnetisation Using Cationised Magnetoferritin

Authors: Correia Carreira, Sara

  • Nominated as an outstanding PhD thesis by the University of Bristol, Bristol, UK
  • Presents a comprehensive introduction to magnetic nanoparticles and their use in magnetic cell labelling Includes a detailed protocol describing the synthesis and functionalisation of novel, protein-based magnetic nanoparticles for ultrafast and universal cell labelling Discusses in-depth the characterisation of magnetic labelling efficiency and the effects on stem cell biology
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eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-60333-9
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Hardcover $129.00
price for USA
  • ISBN 978-3-319-60332-2
  • Free shipping for individuals worldwide
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About this book

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently. 

Table of contents (7 chapters)

  • Introduction

    Correia Carreira, Sara

    Pages 1-44

  • Materials and Methods

    Correia Carreira, Sara

    Pages 45-100

  • Characterising Magnetoferritin and Cationised Magnetoferritin

    Correia Carreira, Sara

    Pages 101-110

  • Stem Cell Labelling with Cationised Magnetoferritin

    Correia Carreira, Sara

    Pages 111-123

  • Toxicological Profiling of Cationised Magnetoferritin

    Correia Carreira, Sara

    Pages 125-137

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-60333-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-60332-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rapid Cell Magnetisation Using Cationised Magnetoferritin
Authors
Series Title
Springer Theses
Copyright
2017
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing AG
eBook ISBN
978-3-319-60333-9
DOI
10.1007/978-3-319-60333-9
Hardcover ISBN
978-3-319-60332-2
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XVII, 166
Number of Illustrations and Tables
74 b/w illustrations, 16 illustrations in colour
Topics