Techniques in Life Science and Biomedicine for the Non-Expert

Elispot for Rookies (and Experts Too)

Authors: Janetzki, Sylvia

  • Provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible
  • Allows the novice user to truly understand the best options for specific protocol steps, reagents and materials
  • Techniques presented are supported by the author’s twenty-plus years of first-hand experience working with this assay
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eBook $89.00
price for USA (gross)
  • ISBN 978-3-319-45295-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.99
price for USA
  • ISBN 978-3-319-45293-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.

The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

About the authors

Sylvia Janetzki is the CEO of the ZellNet Consulting, whose services cover all aspects of Elispot, including troubleshooting, review of protocol, set-up for clinical trial monitoring, certification services, evaluation of plates, and training procedures.

Table of contents (10 chapters)

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-3-319-45295-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.99
price for USA
  • ISBN 978-3-319-45293-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Elispot for Rookies (and Experts Too)
Authors
Series Title
Techniques in Life Science and Biomedicine for the Non-Expert
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-45295-1
DOI
10.1007/978-3-319-45295-1
Hardcover ISBN
978-3-319-45293-7
Series ISSN
2367-1114
Edition Number
1
Number of Pages
VIII, 93
Number of Illustrations and Tables
7 b/w illustrations, 32 illustrations in colour
Topics