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Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

Authors: Till, B.J., Jankowicz-Cieslak, J., Huynh, O.A., Beshir, M.M., Laport, R.G., Hofinger, B.J.

  • Open Access provided with a grant from the International Atomic Energy Agency
  • Describes low-cost and easy-to-use assays for the molecular characterization of mutant plant material
  • The experimental protocols do away with the need for complicated procedures, expensive equipment and hazardous chemicals
  • All protocols included have been tried and tested by researchers all over the world
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Buy this book

eBook  
  • ISBN 978-3-319-16259-1
  • This book is an open access book, you can download it for free on link.springer.com
Hardcover $59.99
price for USA
  • ISBN 978-3-319-16258-4
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
Softcover $59.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 4, 2016
  • ISBN 978-3-319-38667-6
  • Free shipping for individuals worldwide
About this book

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Table of contents (9 chapters)

  • Introduction

    Till, Bradley J. (et al.)

    Pages 1-4

  • Health and Safety Considerations

    Till, Bradley J. (et al.)

    Pages 5-7

  • Sample Collection and Storage

    Till, Bradley J. (et al.)

    Pages 9-11

  • Low-Cost DNA Extraction

    Till, Bradley J. (et al.)

    Pages 13-17

  • PCR Amplification for Low-Cost Mutation Discovery

    Till, Bradley J. (et al.)

    Pages 19-20

Buy this book

eBook  
  • ISBN 978-3-319-16259-1
  • This book is an open access book, you can download it for free on link.springer.com
Hardcover $59.99
price for USA
  • ISBN 978-3-319-16258-4
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
Softcover $59.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 4, 2016
  • ISBN 978-3-319-38667-6
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Low-Cost Methods for Molecular Characterization of Mutant Plants
Book Subtitle
Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Authors
Copyright
2015
Publisher
Springer International Publishing
Copyright Holder
The Editor(s) (if applicable) and the Author(s)
eBook ISBN
978-3-319-16259-1
DOI
10.1007/978-3-319-16259-1
Hardcover ISBN
978-3-319-16258-4
Softcover ISBN
978-3-319-38667-6
Edition Number
1
Number of Pages
X, 35
Number of Illustrations and Tables
6 b/w illustrations, 3 illustrations in colour
Topics