Springer Series on Fluorescence

Advanced Photon Counting

Applications, Methods, Instrumentation

Editors: Kapusta, Peter, Wahl, Michael, Erdmann, Rainer (Eds.)

  • A comprehensive review
  • Written by experts
  • Richly illustrated
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eBook $229.00
price for USA (gross)
  • ISBN 978-3-319-15636-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $349.99
price for USA
  • ISBN 978-3-319-15635-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $299.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 18, 2016
  • ISBN 978-3-319-35429-3
  • Free shipping for individuals worldwide
About this book

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.





About the authors
Reviews

“The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. … In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists.” (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)


Table of contents (16 chapters)

  • Modern TCSPC Electronics: Principles and Acquisition Modes

    Wahl, Michael

    Pages 1-21

  • Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm

    Bülter, Andreas

    Pages 23-42

  • Single-Photon Detectors for Infrared Wavelengths in the Range 1–1.7 μm

    Buller, Gerald S. (et al.)

    Pages 43-69

  • Modern Pulsed Diode Laser Sources for Time-Correlated Photon Counting

    Schönau, Thomas (et al.)

    Pages 71-87

  • Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS

    Dertinger, Thomas (et al.)

    Pages 89-109

Buy this book

eBook $229.00
price for USA (gross)
  • ISBN 978-3-319-15636-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $349.99
price for USA
  • ISBN 978-3-319-15635-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $299.99
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: November 18, 2016
  • ISBN 978-3-319-35429-3
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Advanced Photon Counting
Book Subtitle
Applications, Methods, Instrumentation
Editors
  • Peter Kapusta
  • Michael Wahl
  • Rainer Erdmann
Series Title
Springer Series on Fluorescence
Series Volume
15
Copyright
2015
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-15636-1
DOI
10.1007/978-3-319-15636-1
Hardcover ISBN
978-3-319-15635-4
Softcover ISBN
978-3-319-35429-3
Series ISSN
1617-1306
Edition Number
1
Number of Pages
XII, 370
Number of Illustrations and Tables
56 b/w illustrations, 103 illustrations in colour
Topics