Springer Theses

Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes

Authors: Daumann, Lena

  • Nominated as an Outstanding Ph.D. thesis by the University of Queensland, Australia
  • Encompasses a broad range of topics including biochemical, kinetic and mechanistic studies, chemical synthesis, as well as the use of polymeric and nanoparticle supports
  • Enhances our understanding of hydrolytic cleavage of important substrates like phosphoesters and β-lactams
  • Includes a short introduction to each chapter to guide non-specialist readers
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Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-06629-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-06628-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: October 28, 2016
  • ISBN 978-3-319-36098-0
  • Free shipping for individuals worldwide
About this book

Lena Daumanns's thesis describes structural and functional studies of the enzyme Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. It also examines the properties of small mimics of this enzyme and related binuclear metallohydrolases such as the metallo-ß-lactamases to enhance our understanding of hydrolytic cleavage of important substrates like phosphoesters and β-lactams. Overall, this project has led to a better understanding of the metal ion binding and active site structural features of the enzyme GpdQ. Daumann describes how she successfully immobilized phosphoesterase and related biomimetics on solid supports for potential applications in the area of bioremediation of organophosphate pesticides. Analysis shows that both the enzyme and biomimetics can be stored on the solid support without loss of activity. Furthermore, the author specroscopically and mechanistically characterized a number of Zn(II), Cd(II) and Co(II) complexes, some of which are among the most active biomimetics towards organophosphates reported to date. This thesis makes excellent reading for non-specialists because each chapter includes a short introduction section.

About the authors

Lena Daumann holds a Diploma in Chemistry from the University of Heidelberg, Germany, where she worked with Professor Peter Comba. After gathering six months industrial experience at the BASF chemical company, she commenced her PhD in 2010 at the University of Queensland, Australia, under the supervision of Professor Lawrence Gahan. She received her doctoral degree in 2013 in the field of Bioinorganic Chemistry and currently holds a position as a postdoctoral researcher at the University of California, Berkeley, in the group of Professor Kenneth Raymond, funded by a fellowship of the Humboldt Foundation.

Table of contents (9 chapters)

  • Introduction

    Daumann, Lena Josefine

    Pages 1-12

  • Experimental

    Daumann, Lena Josefine

    Pages 13-64

  • Understanding the Overall Structure of GpdQ and Metal Binding

    Daumann, Lena Josefine

    Pages 65-87

  • Structural and Mechanistic Studies of Zn(II) Complexes as Phosphoesterase Models

    Daumann, Lena Josefine

    Pages 89-117

  • Mechanistic Studies of Cd(II) Complexes as Phosphoesterase and Metallo-β-lactamase Models

    Daumann, Lena Josefine

    Pages 119-143

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-06629-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-06628-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: October 28, 2016
  • ISBN 978-3-319-36098-0
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases and Their Biomimetic Complexes
Authors
Series Title
Springer Theses
Copyright
2014
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-06629-5
DOI
10.1007/978-3-319-06629-5
Hardcover ISBN
978-3-319-06628-8
Softcover ISBN
978-3-319-36098-0
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XXI, 239
Number of Illustrations and Tables
178 b/w illustrations, 22 illustrations in colour
Topics