Springer Theses

A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry

Authors: Wang, Xiaoshi

  • Nominated as an outstanding Ph.D. thesis by Princeton University, USA
  • Represents significant scientific advance in the field of heme-thiolate enzymes
  • Provides clear and colorful illustrations with original research data
  • Give complete and detailed enzymatic function studies and mechanistic frameworks
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eBook $99.00
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  • ISBN 978-3-319-03236-8
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  • Immediate eBook download after purchase
Hardcover $129.00
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  • ISBN 978-3-319-03235-1
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About this book

In this thesis, Xiaoshi Wang investigates the function and mechanism of a newly discovered heme-thiolate peroxygenase, AaeAPO. This enzyme class comes from Agrocybe aegerita and is used in the conversion of inert hydrocarbons to alcohols. Xiaoshi's work focuses on an extracellular P450 enzyme which is not limited in its stability and lack of solubility and therefore is relevant for widespread industrial use. The author demonstrates that the peroxygenase catalyzes a wide range of reactions. In some cases the author even describes very difficult transformations in molecules that are highly inert. Her detailed investigations provide a mechanistic framework for how the peroxygenase catalyzes such a large number of reactions. A major highlight of this thesis is the identification of key short-lived intermediates in the catalytic cycle of the peroxygenase, using rapid kinetic and spectroscopic methods, as well as the elucidation of the thermodynamic properties of these high-energy intermediates. This work adds new insight into an important class of enzymes.

About the authors

Xiaoshi Wang received her B.Sc. in chemistry from Nanjing University, China in 2007. She obtained her Ph.D. in chemistry from Chemistry Department of Princeton University in August 2013. Her major research project in Prof. John T. Groves group is function and mechanistic investigation on C-H oxygenation reactions catalyzed by heme-thiolate enzymes. Subsequently, she became a post-doc fellow working with Prof. Benjamin Garcia at Perelman School of Medicine, University of Pennsylvania, focusing on studying chromatin protein PTMs during epigenetic processes.

Table of contents (7 chapters)

  • Hydrocarbon Oxygenation by Heme-Thiolate Enzymes

    Wang, Xiaoshi

    Pages 1-21

  • Efficient and Selective Alkane Hydroxylation Reactions Catalyzed by the Fungal Peroxygenase AaeAPO

    Wang, Xiaoshi

    Pages 23-40

  • Hydrocarbon Hydroxylations Catalyzed by AaeAPO: Evidence of Radical Intermediates and Kinetic Isotope Effects

    Wang, Xiaoshi

    Pages 41-57

  • Detection and Kinetic Characterization of a Highly Reactive Heme-Thiolate Peroxygenase AaeAPO Compound I

    Wang, Xiaoshi

    Pages 59-74

  • Driving Force for Oxygen Atom Transfer by Heme-Thiolate Enzymes

    Wang, Xiaoshi

    Pages 75-89

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-03236-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-03235-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry
Authors
Series Title
Springer Theses
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-03236-8
DOI
10.1007/978-3-319-03236-8
Hardcover ISBN
978-3-319-03235-1
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XXXII, 130
Topics