Springer Theses

Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo

Authors: Sharp, Thomas H.

  • A concise primer to a variety of both light and electron microscopy techniques allows the reader to make an informed decision regarding which one will be best for their own research
  • An expansive introduction to the analysis of electron microscopy images prepares the reader for a variety of structure determination procedures
  • Each chapter starts with an overview of the material covered
  • Attractive layout with hyperlinks to related sections, figures and references creates a format ideal for quick and easy referencing
  • Full methods section explains how to perform each technique covered
  • Comprehensive explanations and over 80 illustrations lead the reader through the results presented
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Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-02159-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-02158-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: October 6, 2016
  • ISBN 978-3-319-35023-3
  • Free shipping for individuals worldwide
About this book

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.

About the authors

Thom Sharp completed his bachelor’s degree in Biochemistry at the University of Bristol, with a year spent a year working at AstraZeneca. He continued in Bristol for his PhD in bionanotechnology, which was performed under the supervision of Dek Woolfson, a protein design chemist, and Paul Verkade, an electron microscopist. Thom is currently at the University of Oxford, where he is developing DNA-templated protein arrays to increase the throughput of single-particle analysis.

Table of contents (5 chapters)

  • Biomolecules and Microscopy

    Sharp, Thomas Harry

    Pages 3-56

  • Experimental Methods

    Sharp, Thomas Harry

    Pages 59-72

  • Imaging Methods

    Sharp, Thomas Harry

    Pages 73-82

  • Towards an Atomistic Structure of a De Novo Designed Peptide Fibre

    Sharp, Thomas Harry

    Pages 85-110

  • Developing Novel Probes for Correlative Light Electron Microscopy

    Sharp, Thomas Harry

    Pages 111-143

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-02159-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-02158-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • Customers within the U.S. and Canada please contact Customer Service at 1-800-777-4643, Latin America please contact us at +1-212-460-1500 (Weekdays 8:30am – 5:30pm ET) to place your order.
  • Due: October 6, 2016
  • ISBN 978-3-319-35023-3
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo
Authors
Series Title
Springer Theses
Copyright
2014
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-02159-1
DOI
10.1007/978-3-319-02159-1
Hardcover ISBN
978-3-319-02158-4
Softcover ISBN
978-3-319-35023-3
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XVIII, 150
Number of Illustrations and Tables
30 b/w illustrations, 55 illustrations in colour
Topics