Lecture Notes in Chemistry

Laser Photodissociation and Spectroscopy of Mass-separated Biomolecular Ions

Editors: Polfer, Nicolas C., Dugourd, Philippe (Eds.)

  • Edited by two renowned experts for UV-vis and IR laser based photodissociation and spectroscopy in MS of biomolecules
  • Introduces methods combining mass spectrometry (MS) and spectroscopy - a particularly powerful combination for enhanced structural information on biomolecules
  • Explains the basics, from fundamentals about spectroscopy over tunable light sources to ion trapping devices
  • Demonstrates the power of spectroscopic interrogation of biomolecules, from peptides, over proteins, to saccharides; in the present state-of-the-art, and in an outlook on future developments
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eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-01252-0
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  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-01251-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34572-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This lecture notes book presents how enhanced structural information of biomolecular ions can be obtained from interaction with photons of specific frequency - laser light. The methods described in the book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" make use of the fact that the discrete energy and fast time scale of photoexcitation can provide more control in ion activation. This activation is the crucial process producing structure-informative product ions that cannot be generated with more conventional heating methods, such as collisional activation. The book describes how the powerful separation capabilities and sensitivity of mass spectrometry (MS) can be combined with the structural insights from spectroscopy by measuring vibrational and electronic spectra of trapped analytes.  The implementation of laser-based photodissociation techniques in MS requires basic knowledge of tunable light sources and ion trapping devices.

This book introduces the reader to key concepts and approaches in molecular spectroscopy, and the light sources and ion traps employed in such experiments. The power of the methods is demonstrated by spectroscopic interrogation of a range of important biomolecular systems, including peptides, proteins, and saccharides, with laser light in the ultraviolet-visible, and infrared range.  The book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" is an indispensable resource for students and researchers engaged or interested in this emerging field. It provides the solid background of key concepts and technologies for the measurements, discusses state-of-the-art experiments, and provides an outlook on future developments and applications.

Table of contents (5 chapters)

  • Spectroscopy and the Electromagnetic Spectrum

    Stedwell, Corey N. (et al.)

    Pages 1-20

  • Light Sources

    Roehr, Nathan P. (et al.)

    Pages 21-47

  • Ion Traps

    Gulyuz, Kerim (et al.)

    Pages 49-70

  • Infrared Photodissociation of Biomolecular Ions

    Polfer, Nicolas C. (et al.)

    Pages 71-91

  • UV-Visible Activation of Biomolecular Ions

    Antoine, Rodolphe (et al.)

    Pages 93-116

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-01252-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-01251-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34572-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Laser Photodissociation and Spectroscopy of Mass-separated Biomolecular Ions
Editors
  • Nicolas C. Polfer
  • Philippe Dugourd
Series Title
Lecture Notes in Chemistry
Series Volume
83
Copyright
2013
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-01252-0
DOI
10.1007/978-3-319-01252-0
Hardcover ISBN
978-3-319-01251-3
Softcover ISBN
978-3-319-34572-7
Series ISSN
0342-4901
Edition Number
1
Number of Pages
XI, 119
Number of Illustrations and Tables
43 b/w illustrations, 36 illustrations in colour
Topics