Springer Theses

Purification of Peptides in High-Complexity Arrays

A New Method for the Specific Surface Exchange and Purification of Entire Peptide Libraries

Authors: Schirwitz, Christopher

  • Nominated as an outstanding Ph.D. thesis by the Institute of Applied Physical Chemistry, University of Heidelberg, and the German Cancer Research Center (DKFZ) Heidelberg, Germany
  • Outlines an improvement of the quality of in situ synthesized high-complexity peptide micro arrays
  • Highlights the development of a new method allowing for fast one-step purification of entire arrays without loss of resolution or spatial information
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eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-00807-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-00806-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34559-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Christopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research.
With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide
micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research
Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire
arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.

Table of contents (5 chapters)

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-00807-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-00806-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34559-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Purification of Peptides in High-Complexity Arrays
Book Subtitle
A New Method for the Specific Surface Exchange and Purification of Entire Peptide Libraries
Authors
Series Title
Springer Theses
Copyright
2013
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-00807-3
DOI
10.1007/978-3-319-00807-3
Hardcover ISBN
978-3-319-00806-6
Softcover ISBN
978-3-319-34559-8
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XIII, 132
Number of Illustrations and Tables
6 b/w illustrations, 53 illustrations in colour
Topics