Overview
- Nominated as an outstanding Ph.D. thesis by the Institute of Applied Physical Chemistry, University of Heidelberg, and the German Cancer Research Center (DKFZ) Heidelberg, Germany
- Outlines an improvement of the quality of in situ synthesized high-complexity peptide micro arrays
- Highlights the development of a new method allowing for fast one-step purification of entire arrays without loss of resolution or spatial information
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (5 chapters)
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Front Matter
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Back Matter
Keywords
About this book
With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide
micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research
Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire
arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
Authors and Affiliations
Bibliographic Information
Book Title: Purification of Peptides in High-Complexity Arrays
Book Subtitle: A New Method for the Specific Surface Exchange and Purification of Entire Peptide Libraries
Authors: Christopher Schirwitz
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-3-319-00807-3
Publisher: Springer Cham
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer International Publishing Switzerland 2013
Hardcover ISBN: 978-3-319-00806-6Published: 21 August 2013
Softcover ISBN: 978-3-319-34559-8Published: 23 August 2016
eBook ISBN: 978-3-319-00807-3Published: 13 August 2013
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XIII, 132
Number of Illustrations: 6 b/w illustrations, 53 illustrations in colour
Topics: Bioorganic Chemistry, Protein Science, Physical Chemistry