Springer Theses

How Free Cationic Polymer Chains Promote Gene Transfection

Authors: Yanan, Yue

  • Nominated as an outstanding Ph.D. thesis by the Chinese University of Hong Kong
  • Addresses and elucidates a critical but long overlooked question on non-viral gene delivery
  • Greatly deepens our understanding of polymer-mediated gene transfection, representing a paradigm shift
  • Offers new insights into the rational design of next-generation superior polymeric vectors for gene delivery
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eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-00336-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-00335-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34378-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

Table of contents (4 chapters)

  • Introduction and Background

    Yanan, Yue

    Pages 1-27

  • Revisiting Complexation Between DNA and Polyethylenimine: The Effect of Uncomplexed Chains Free in the Solution Mixture on Gene Transfection

    Yanan, Yue

    Pages 29-48

  • Revisiting Complexation Between DNA and Polyethylenimine: The Effect of Length of Free Polycationic Chains on Gene Transfection

    Yanan, Yue

    Pages 49-72

  • Quantitative Comparison of Endocytosis and Intracellular Trafficking of DNA/Polymer Complexes in the Absence/Presence of Free Polycationic Chains

    Yanan, Yue

    Pages 73-94

Buy this book

eBook $99.00
price for USA (gross)
  • ISBN 978-3-319-00336-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $129.00
price for USA
  • ISBN 978-3-319-00335-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $129.00
price for USA
  • ISBN 978-3-319-34378-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
How Free Cationic Polymer Chains Promote Gene Transfection
Authors
Series Title
Springer Theses
Copyright
2013
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-00336-8
DOI
10.1007/978-3-319-00336-8
Hardcover ISBN
978-3-319-00335-1
Softcover ISBN
978-3-319-34378-5
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XVIII, 94
Number of Illustrations and Tables
7 b/w illustrations, 38 illustrations in colour
Topics