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Methods in Molecular Biology

Riboswitches

Methods and Protocols

Editors: Serganov, Alexander (Ed.)

  • A comprehensive and timely reference volume for basic and applied research on regulatory RNAs
  • Covers a wide range of assay formats and systems from the computerized prediction of regulatory RNA elements to their detailed structure-function characterization
  • Focuses on both natural and synthetic RNA molecules
  • Provides step-by-step protocols of rigorously tested classical and cutting-edge in vivo and in vitro methods
  • Presents techniques easily adaptable for experiments with any RNA species
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-558-9
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-934115-88-6
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-947-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues.  In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control.  Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology.

Reviews

From the reviews:

"Riboswitches are among the latest additions to the growing field of RNA-based gene expression control systems found in both prokaryotic and eukaryotic cells. … useful in various aspects of work on riboswitches and other regulatory RNAs. … It also refers to pertinent and up-to-date literature. … The reviewed book can be strongly recommended to RNA researchers with different levels of expertise, as well as to those who learn or teach in this field." (Joanna Ciesla, Acta Biochimica Polonica, Vol. 56 (3), 2009)

“This book is a good collection of ‘Methods and Protocols’ for studying … riboswitches as well as some other RNAs involved in gene expression. … Readers are introduced to a broad range of RNA methodologies from basic to applied in this book. I … recommend the book for researchers who work in the area of riboswitches, but also for other scientists who are involved in RNA-relevant studies.” (Atsushi Ogawa, ChemBioChem, Vol. 10, 2009)

Table of contents (25 chapters)

  • Predicting Riboswitch Regulation on a Genomic Scale

    Barrick, Jeffrey E.

    Pages 1-13

  • Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications

    Rieder, Renate (et al.)

    Pages 15-24

  • Application of Fluorescent Measurements for Characterization of Riboswitch–Ligand Interactions

    Heppell, Benoit (et al.)

    Pages 25-37

  • Transcriptional Approaches to Riboswitch Studies

    Mironov, Alexander (et al.)

    Pages 39-51

  • Kinetics of Riboswitch Regulation Studied By In Vitro Transcription

    Wickiser, J. Kenneth

    Pages 53-63

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-558-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-934115-88-6
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-947-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Riboswitches
Book Subtitle
Methods and Protocols
Editors
  • Alexander Serganov
Series Title
Methods in Molecular Biology
Series Volume
540
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-558-9
DOI
10.1007/978-1-59745-558-9
Hardcover ISBN
978-1-934115-88-6
Softcover ISBN
978-1-61737-947-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 366
Number of Illustrations and Tables
65 b/w illustrations
Topics