Methods in Molecular Biology

Monoclonal Antibodies

Methods and Protocols

Editors: Ossipow, Vincent, Fischer, Nicolas (Eds.)

  • Offers modern approachse to generate high quality monoclonals
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-992-5
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Hardcover $139.00
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  • ISBN 978-1-62703-991-8
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Softcover $139.00
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  • ISBN 978-1-4939-6322-5
  • Free shipping for individuals worldwide
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About this book

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

Table of contents (36 chapters)

  • Antigen Production for Monoclonal Antibody Generation

    Magistrelli, Giovanni (et al.)

    Pages 3-20

  • Method for the Generation of Antibodies Specific for Site and Posttranslational Modifications

    Goto, Hidemasa (et al.)

    Pages 21-31

  • Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production

    Kim, Hyung-Yong (et al.)

    Pages 33-45

  • Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion

    Chronopoulou, Efthalia (et al.)

    Pages 47-70

  • Generation of Rabbit Monoclonal Antibodies

    Yam, Pi-Chen (et al.)

    Pages 71-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-992-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-991-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6322-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Monoclonal Antibodies
Book Subtitle
Methods and Protocols
Editors
  • Vincent Ossipow
  • Nicolas Fischer
Series Title
Methods in Molecular Biology
Series Volume
1131
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-62703-992-5
DOI
10.1007/978-1-62703-992-5
Hardcover ISBN
978-1-62703-991-8
Softcover ISBN
978-1-4939-6322-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 575
Number of Illustrations and Tables
81 b/w illustrations, 27 illustrations in colour
Topics