Methods in Molecular Biology

Spliceosomal Pre-mRNA Splicing

Methods and Protocols

Editors: Hertel, Klemens J. (Ed.)

  • Introduces protocols and their theoretical background relevant for a variety of experimental research in pre-mRNA splicing
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-980-2
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Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-979-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6044-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.

Table of contents (28 chapters)

  • The Pre-mRNA Splicing Reaction

    Wongpalee, Somsakul Pop (et al.)

    Pages 3-12

  • Diversity and Evolution of Spliceosomal Systems

    Roy, Scott William (et al.)

    Pages 13-33

  • Mechanisms of Spliceosomal Assembly

    Chiou, Ni-ting (et al.)

    Pages 35-43

  • Alternative Pre-mRNA Splicing

    Wagner, Stacey D. (et al.)

    Pages 45-54

  • Regulation of Alternative Pre-mRNA Splicing

    Coelho, Miguel B. (et al.)

    Pages 55-82

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-980-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-979-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6044-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Spliceosomal Pre-mRNA Splicing
Book Subtitle
Methods and Protocols
Editors
  • Klemens J. Hertel
Series Title
Methods in Molecular Biology
Series Volume
1126
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-980-2
DOI
10.1007/978-1-62703-980-2
Hardcover ISBN
978-1-62703-979-6
Softcover ISBN
978-1-4939-6044-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 427
Number of Illustrations and Tables
32 b/w illustrations, 34 illustrations in colour
Topics