Methods in Molecular Biology

Homing Endonucleases

Methods and Protocols

Editors: Edgell, David (Ed.)

  • ​​Serves as a comprehensive resource to identify and characterize homing endonucleases and much more
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-968-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-967-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6319-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.

Table of contents (17 chapters)

  • Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers

    Belfort, Marlene (et al.)

    Pages 1-26

  • Bioinformatic Identification of Homing Endonucleases and Their Target Sites

    Privman, Eyal

    Pages 27-35

  • PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes

    Hafez, Mohamed (et al.)

    Pages 37-53

  • Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein

    Bonocora, Richard P. (et al.)

    Pages 55-67

  • Mapping Free-Standing Homing Endonuclease Promoters Using 5′RLM-RACE

    Gibb, Ewan A.

    Pages 69-76

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-968-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-967-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6319-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Homing Endonucleases
Book Subtitle
Methods and Protocols
Editors
  • David Edgell
Series Title
Methods in Molecular Biology
Series Volume
1123
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-968-0
DOI
10.1007/978-1-62703-968-0
Hardcover ISBN
978-1-62703-967-3
Softcover ISBN
978-1-4939-6319-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 284
Number of Illustrations and Tables
21 b/w illustrations, 39 illustrations in colour
Topics